PMID- 17259144
OWN - NLM
STAT- MEDLINE
DCOM- 20080512
LR  - 20161124
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 27
IP  - 1
DP  - 2007 Jan
TI  - [Effect of superparamagnetic iron oxide labeling on neural stem cell survival and 
      proliferation].
PG  - 49-51, 55
AB  - OBJECTIVE: To study the effect of superparamgnetic iron oxides (ferumoxides) on 
      the survival and proliferation of neural stem cells (NSCs) and determine the 
      optimal ferumoxides concentration for labeling. METHODS: Bone marrow stromal 
      cells (BMSCs) were obtained from rat femoral marrow and cultured in vitro to 
      induce their differentiation into NSCs. Ferumoxides labeling of the NSCs was 
      performed with different final concentrations of ferumoxides, and the labeling 
      efficiency and viability of the labeled NSCs were evaluated by Prussian blue 
      staining, MTT assay, flow cytometry and transmission electron microscope. 
      RESULTS: The NSCs could be effectively labeled with ferumoxides with a labeling 
      efficiency of around 90%. Prussian blue staining showed numerous fine granules 
      with blue staining in the cytoplasm of the labeled NSCs, and the intensity of the 
      blue staining was in positive correlation with the ferumoxide concentration for 
      labeling. Transmission electron microscopy of the labeled NSCs revealed the 
      presence of numerous vesicles spreading in the cytoplasm and filled with 
      electron-dense magnetic iron particles. The ferumoxides vesicles increased with 
      the labeling concentration of ferumoxides, and at the final concentration 
      exceeding 25 microg/ml, ferumoxides vesicles in the NSCs gave rise to 
      conglomeration which hampered observation of the cellular ultrastructure by 
      transmission electron microscope. The results of flow cytometry and MTT assay 
      demonstrated that the cell viability, proliferation, differentiation and 
      apoptosis of the labeled cells were affected by ferumoxides at the concentration 
      above 25 microg/ml, but such effects could be minimal at lower concentrations. 
      CONCLUSION: Ferumoxides might be feasible for in vitro labeling of the NSCs with 
      the optimal concentration of 25 microg/ml.
FAU - Dai, Guang-Hui
AU  - Dai GH
AD  - Institute of Neuromedicine, Zhujiang Hospital, Southern Medical University, 
      Guangzhou 510282, China. daig74@126.com
FAU - Xiu, Jun-Gang
AU  - Xiu JG
FAU - Zhou, Zhen-Jun
AU  - Zhou ZJ
FAU - Chen, Zhong-Can
AU  - Chen ZC
FAU - Xu, Ru-Xiang
AU  - Xu RX
FAU - Jiang, Xiao-Dan
AU  - Jiang XD
FAU - Du, Mou-Xuan
AU  - Du MX
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Dextrans)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Oxides)
RN  - E1UOL152H7 (Iron)
RN  - G6N3J05W84 (ferumoxides)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/*drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Dextrans
MH  - Ferrosoferric Oxide
MH  - Iron/*pharmacology
MH  - Magnetite Nanoparticles
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Neurons/*cytology/ultrastructure
MH  - Oxides/*pharmacology
MH  - Rats
MH  - Stem Cells/*cytology/ultrastructure
EDAT- 2007/01/30 09:00
MHDA- 2008/05/13 09:00
CRDT- 2007/01/30 09:00
PHST- 2007/01/30 09:00 [pubmed]
PHST- 2008/05/13 09:00 [medline]
PHST- 2007/01/30 09:00 [entrez]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2007 Jan;27(1):49-51, 55.