PMID- 17260792 OWN - NLM STAT- MEDLINE DCOM- 20071120 LR - 20161124 IS - 1001-5302 (Print) IS - 1001-5302 (Linking) VI - 31 IP - 21 DP - 2006 Nov TI - [Simultaneous determination of eight organic acids in Fructus mume by RP-HPLC]. PG - 1783-6 AB - OBJECTIVE: To develop an HPLC method for the simultaneous separation and determination of oxalic acid (OA), tartaric acid(TA), malic acid(MA), vitamin C (VC), lactic acid (LA), acetic acid (AA) citric acid (CA) and succinic acid (SA) in Fructus mume. METHOD: Analytical column was Zorbax Eclipse XDB C18. Mobile phase was 0.5% (NH4) H2PO4 aqueous solution and detection wavelength was 214 nm. The flow rate of mobile phase was 0.5 mL x min(-1). RESULT: The regression equations (pH 2. 8, adjusted with phosphoric acid) of eight constituents have been established, r = 0.999 7, 0. 999 8, 0.999 2, 0.999 6, 0.999 1, 0.999 5, 0.999 8, 0.999 2 respectively. Meanwhile, the content and proportion relationship of eight organic acids in Fructus mume which yielded in Fujian (China) were investigated. CONCLUSION: This method was simple, accuracy and quick. The method can be used for the purpose of routine analysis and the quality control of a botanic (Fructus mume) containing these organic acid components. FAU - Chen, Zhan-Guo AU - Chen ZG AD - College of Chemistry and Materials Science, Shaaxi Normal University, Xi'an 710062, China. chzhg@snnu.edu.cn FAU - En, Bo-Ti AU - En BT FAU - Zhang, Zhi-Qi AU - Zhang ZQ LA - chi PT - Journal Article PL - China TA - Zhongguo Zhong Yao Za Zhi JT - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JID - 8913656 RN - 0 (Carboxylic Acids) RN - 0 (Malates) RN - 0 (Tartrates) RN - 2968PHW8QP (Citric Acid) RN - 33X04XA5AT (Lactic Acid) RN - 817L1N4CKP (malic acid) RN - 9E7R5L6H31 (Oxalic Acid) RN - AB6MNQ6J6L (Succinic Acid) RN - PQ6CK8PD0R (Ascorbic Acid) RN - Q40Q9N063P (Acetic Acid) RN - W4888I119H (tartaric acid) SB - IM MH - Acetic Acid/analysis MH - Ascorbic Acid/analysis MH - Carboxylic Acids/*analysis MH - Chromatography, High Pressure Liquid/methods MH - Citric Acid/analysis MH - Fruit/chemistry MH - Lactic Acid/analysis MH - Malates/analysis MH - Oxalic Acid/analysis MH - Plants, Medicinal/*chemistry MH - Prunus/*chemistry MH - Quality Control MH - Reproducibility of Results MH - Succinic Acid/analysis MH - Tartrates/analysis EDAT- 2007/01/31 09:00 MHDA- 2007/12/06 09:00 CRDT- 2007/01/31 09:00 PHST- 2007/01/31 09:00 [pubmed] PHST- 2007/12/06 09:00 [medline] PHST- 2007/01/31 09:00 [entrez] PST - ppublish SO - Zhongguo Zhong Yao Za Zhi. 2006 Nov;31(21):1783-6.