PMID- 17261135
OWN - NLM
STAT- MEDLINE
DCOM- 20070607
LR  - 20211203
IS  - 1751-2972 (Print)
IS  - 1751-2972 (Linking)
VI  - 8
IP  - 1
DP  - 2007 Feb
TI  - Effect of a novel phyto-compound on mucosal candidiasis: further evidence from an 
      ex vivo study.
PG  - 48-51
AB  - OBJECTIVE: To isolate, identify and determine the prevalence of yeasts in the 
      oral cavity of individuals and to test the minimum inhibitory dilution (MID) of 
      Kolorex against the yeasts isolated. METHODS: Twenty-nine individuals of both 
      sexes aged on average 61.3 years were evaluated at the dental clinic in order to 
      isolate and identify yeasts from their oral cavity, with and without lesions, and 
      to determine the MID of the commercial phyto-product Kolorex against the strains 
      isolated. The antifungal activity of the product tested was determined by the 
      technique of dilution on a solid medium. Monocyte chemoattractant protein 1 
      (MCP-1) was measured by biotinylated antibody assay by an enzyme-linked 
      immunosorbent assay (ELISA). RESULTS: Yeasts of the genus Candida were detected 
      in the saliva of 45.4% of the 11 individuals with a clinically healthy mouth and 
      in 88.2% of 17 individuals with oral lesions. In the group with oral candidiasis 
      we isolated in tongue and lesion, respectively, for each species: C. tropicalis 
      (5.8% and 11.7%), C. glabrata (5.8% and 5.8%) and C. parapsilosis (0% and 5.8%), 
      in addition to C. albicans as the only species or in association with others, 
      respectively (64.7% and 70.5%). The total clonal formation unit (CFU) (counts/mL) 
      in the saliva showed a higher mean value in the group with oral candidiasis 
      (158.3x10(3)) than in the control group (64.6x10(3)). Most of the 70 test strains 
      (95.7%) were sensitive to Kolorex by presenting a MID of 1:20. Sixty percent of 
      strains from the 70 healthy sites showed results similar to those obtained with 
      strains from oral lesions. Different results were mainly observed among different 
      species. Patients with oral lesions showed a significant time-course increase of 
      the level of monocyte chemoattractant protein 1 (MCP 1) as compared to those 
      without lesions or to healthy people in whom Candida has not been detected 
      (P<0.05). Co-culture with Kolorex using aliquots from the same patients with oral 
      lesions inhibited such event (P<0.05). CONCLUSION: Although this study was 
      focused on oral cavity candidiasis, the results indicate the possibility of a 
      broader use of the antifungal Kolorex in the prevention and treatment of mucosal 
      candidiasis located elsewhere.
FAU - Nakajima, Juntaro
AU  - Nakajima J
AD  - Nakajima Dental Clinic, Kobe, Japan.
FAU - Papaah, Pete
AU  - Papaah P
FAU - Yoshizawa, Masako
AU  - Yoshizawa M
FAU - Marotta, Francesco
AU  - Marotta F
FAU - Nakajima, Teriyuki
AU  - Nakajima T
FAU - Mihara, Shin
AU  - Mihara S
FAU - Minelli, Emilio
AU  - Minelli E
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - J Dig Dis
JT  - Journal of digestive diseases
JID - 101302699
RN  - 0 (Allylbenzene Derivatives)
RN  - 0 (Anisoles)
RN  - 0 (Antifungal Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Plant Preparations)
RN  - 0 (Sesquiterpenes)
RN  - 5FAF7T66M7 (polygodial)
RN  - Q3JEK5DO4K (anethole)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Allylbenzene Derivatives
MH  - Anisoles/*pharmacology
MH  - Antifungal Agents/*pharmacology
MH  - Candida/*drug effects
MH  - Candidiasis, Oral/metabolism/*microbiology
MH  - Chemokine CCL2/metabolism
MH  - Female
MH  - Humans
MH  - In Vitro Techniques
MH  - Male
MH  - Microbial Sensitivity Tests
MH  - Middle Aged
MH  - Plant Preparations/*pharmacology
MH  - Sesquiterpenes/*pharmacology
EDAT- 2007/01/31 09:00
MHDA- 2007/06/08 09:00
CRDT- 2007/01/31 09:00
PHST- 2007/01/31 09:00 [pubmed]
PHST- 2007/06/08 09:00 [medline]
PHST- 2007/01/31 09:00 [entrez]
AID - CDD283 [pii]
AID - 10.1111/j.1443-9573.2007.00283.x [doi]
PST - ppublish
SO  - J Dig Dis. 2007 Feb;8(1):48-51. doi: 10.1111/j.1443-9573.2007.00283.x.