PMID- 17265871
OWN - NLM
STAT- MEDLINE
DCOM- 20070227
LR  - 20230307
IS  - 0362-028X (Print)
IS  - 0362-028X (Linking)
VI  - 70
IP  - 1
DP  - 2007 Jan
TI  - Adhesive and chemokine stimulatory properties of potentially probiotic 
      Lactobacillus strains.
PG  - 125-34
AB  - Five Lactobacillus plantarum strains and two Lactobacillus johnsonii strains, 
      stemming either from African traditionally fermented milk products or children's 
      feces, were investigated for probiotic properties in vitro. The relationship 
      between the hydrophobic-hydrophilic cell surface and adhesion ability to HT29 
      intestinal epithelial cells was investigated, and results indicated that 
      especially the L. johnsonii strains, which exhibited both hydrophobic and 
      hydrophilic surface characteristics, adhered well to HT29 cells. Four L. 
      plantarum and two L. johnsonii strains showed high adherence to HT29 cells, 
      generally higher than that of the probiotic control strain Lactobacillus 
      rhamnosus GG. Most strains with high adhesion ability also showed high 
      autoaggregation ability. The two L. johnsonii strains coaggregated well with the 
      intestinal pathogens Listeria monocytogenes Scott A, Staphylococcus aureus ATCC 
      25923, Escherichia coli ATCC 25922, and Salmonella enterica serovar Typhimurium 
      ATCC 14028. The L. plantarum BFE 1685 and L. johnsonii 6128 strains furthermore 
      inhibited the adhesion of at least two of these intestinal pathogens in coculture 
      with HT29 cells in a strain-dependent way. These two potential probiotic strains 
      also significantly increased interleukin-8 (IL-8) chemokine production by HT29 
      cells, although modulation of other cytokines, such as IL-1, IL-6, IL-10, 
      monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor alpha 
      (TNF-alpha), and transforming growth factor beta (TGF-beta), did not occur. 
      Altogether, our results suggested that L. plantarum BFE 1685 and L. johnsonii BFE 
      6128 showed good adherence, coaggregated with pathogens, and stimulated chemokine 
      production of intestinal epithelial cells, traits that may be considered 
      promising for their development as probiotic strains.
FAU - Vizoso Pinto, Maria G
AU  - Vizoso Pinto MG
AD  - Institute of Hygiene and Toxicology, Federal Research Centre for Nutrition and 
      Food, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.
FAU - Schuster, Tobias
AU  - Schuster T
FAU - Briviba, Karlis
AU  - Briviba K
FAU - Watzl, Bernhard
AU  - Watzl B
FAU - Holzapfel, Wilhelm H
AU  - Holzapfel WH
FAU - Franz, Charles M A P
AU  - Franz CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - 0 (Chemokines)
SB  - IM
MH  - Bacterial Adhesion
MH  - Chemokines/*biosynthesis
MH  - Coculture Techniques
MH  - Escherichia coli/physiology
MH  - HT29 Cells/*microbiology
MH  - Humans
MH  - Intestinal Mucosa/metabolism/microbiology
MH  - Lactobacillus/*physiology
MH  - Lactobacillus plantarum/physiology
MH  - Lacticaseibacillus rhamnosus/physiology
MH  - Listeria monocytogenes/physiology
MH  - *Probiotics
MH  - Salmonella typhimurium/physiology
MH  - Species Specificity
MH  - Staphylococcus aureus/physiology
EDAT- 2007/02/03 09:00
MHDA- 2007/02/28 09:00
CRDT- 2007/02/03 09:00
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/02/28 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - S0362-028X(22)05881-1 [pii]
AID - 10.4315/0362-028x-70.1.125 [doi]
PST - ppublish
SO  - J Food Prot. 2007 Jan;70(1):125-34. doi: 10.4315/0362-028x-70.1.125.