PMID- 17266273
OWN - NLM
STAT- MEDLINE
DCOM- 20070502
LR  - 20131121
IS  - 1520-6106 (Print)
IS  - 1520-5207 (Linking)
VI  - 111
IP  - 5
DP  - 2007 Feb 8
TI  - Carbon-nanotube-enhanced direct electron-transfer reactivity of hemoglobin 
      immobilized on polyurethane elastomer film.
PG  - 1182-8
AB  - In this study, we investigate the direct electron-transfer reactivity of 
      immobilized hemoglobin (Hb) on a polyurethane elastomer (PUE) film for biosensor 
      designs. The PUE film synthesized by an additional polymerization possesses good 
      biocompatibility, uniformity, and conformability and is ready for protein 
      immobilization. Electrochemical and spectroscopic measurements show that the 
      presence of multiwalled carbon nanotubes (MWNTs) increased the protein-PUE 
      interaction, varied polymer morphology, improved the permeability and the 
      conductivity of the PUE film, and thus facilitated the direct electron transfer 
      between the immobilized Hb and the conductivity surface through the conducting 
      tunnels of MWNTs. The immobilized Hb maintains its bioactivities and displays an 
      excellent electrochemical behavior with a formal potential of -(334 +/- 7) mV. 
      The addition of NaNO2 leads to an increase of the electrocatalytic reduction 
      current of nitrite at -0.7 V. This allows us to develop a nitrite sensor with a 
      linear response range from 0.08 to 3.6 mM. The proposed method opens a way to 
      develop biosensors by using nanostructured materials mixed with low electrical 
      conductivity matrixes.
FAU - Liu, Songqin
AU  - Liu S
AD  - Department of Chemistry and Chemical Engineering, Southeast University, Nanjing 
      210096, People's Republic of China. liusq@seu.edu.cn
FAU - Lin, Baoping
AU  - Lin B
FAU - Yang, Xiaodi
AU  - Yang X
FAU - Zhang, Qianqian
AU  - Zhang Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Phys Chem B
JT  - The journal of physical chemistry. B
JID - 101157530
RN  - 0 (Elastomers)
RN  - 0 (Hemoglobins)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Polyurethanes)
RN  - S7G510RUBH (Nitrogen Dioxide)
SB  - IM
MH  - Biosensing Techniques/instrumentation/methods
MH  - Elastomers/*chemistry
MH  - Electrochemistry
MH  - Electrodes
MH  - Electron Transport
MH  - Hemoglobins/*chemistry
MH  - *Membranes, Artificial
MH  - Molecular Structure
MH  - Nanotubes, Carbon/*chemistry
MH  - Nitrogen Dioxide/analysis
MH  - Particle Size
MH  - Polyurethanes/chemical synthesis/*chemistry
MH  - Structure-Activity Relationship
MH  - Surface Properties
MH  - Time Factors
EDAT- 2007/02/03 09:00
MHDA- 2007/05/03 09:00
CRDT- 2007/02/03 09:00
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/05/03 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - 10.1021/jp065344b [doi]
PST - ppublish
SO  - J Phys Chem B. 2007 Feb 8;111(5):1182-8. doi: 10.1021/jp065344b.