PMID- 17267564
OWN - NLM
STAT- MEDLINE
DCOM- 20070316
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 27
IP  - 5
DP  - 2007 Jan 31
TI  - Endogenous brain-derived neurotrophic factor triggers fast calcium transients at 
      synapses in developing dendrites.
PG  - 1097-105
AB  - Brain-derived neurotrophic factor (BDNF) is involved in many aspects of the 
      formation of functional neuronal networks. BDNF signaling regulates neuronal 
      development not only globally, at the level of entire neurons or networks, but 
      also at a subcellular level and with high temporal specificity; however, the 
      spatiotemporal characteristics of intrinsic BDNF signaling are essentially 
      unknown. Here, we used calcium imaging to directly observe intrinsic BDNF 
      signaling in developing hippocampal neurons. We found that blocking intrinsic 
      BDNF signaling with function-blocking BDNF antibodies (alphaBDNF) or K252-a 
      reduced the frequency of spontaneously occurring fast and localized calcium rises 
      in dendrites. Conversely, focal application of BDNF evoked fast and local 
      dendritic calcium transients, which required activation of TrkB 
      (tropomyosin-related kinase B) receptors as well as activation of voltage-gated 
      sodium and calcium channels. Virus-mediated expression of PSD-95:CFP 
      (postsynaptic density-95 tagged with cyan fluorescent protein) revealed that 
      spontaneous local calcium transients occurred frequently at postsynaptic sites 
      along the dendrite. The frequency of synaptically localized calcium transients 
      was specifically reduced by blocking intrinsic BDNF signaling, whereas 
      nonsynaptic calcium rises were not affected. Furthermore, focal BDNF delivery 
      evoked localized and fast calcium elevations specifically at postsynaptic sites. 
      Together, our results demonstrate that BDNF-dependent calcium signaling in 
      developing hippocampal neurons is fast and occurs at synapses. These temporal and 
      spatial characteristics of intrinsic BDNF signaling as well as its relative 
      abundance renders BDNF an ideal signaling molecule in the establishment of 
      specific synaptic connectivity and functional neuronal networks.
FAU - Lang, Susanne B
AU  - Lang SB
AD  - Max Planck Institute of Neurobiology, 82152 Martinsried-Munchen, Germany.
FAU - Stein, Valentin
AU  - Stein V
FAU - Bonhoeffer, Tobias
AU  - Bonhoeffer T
FAU - Lohmann, Christian
AU  - Lohmann C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/metabolism/*physiology
MH  - Calcium/physiology
MH  - Calcium Signaling/*physiology
MH  - Cell Line
MH  - Dendrites/*physiology
MH  - Hippocampus/growth & development/metabolism
MH  - Humans
MH  - Neurons/metabolism/physiology
MH  - Organ Culture Techniques
MH  - Pyramidal Cells/metabolism/physiology
MH  - Rats
MH  - Synapses/*physiology
PMC - PMC6673203
EDAT- 2007/02/03 09:00
MHDA- 2007/03/17 09:00
PMCR- 2007/07/31
CRDT- 2007/02/03 09:00
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/03/17 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
PHST- 2007/07/31 00:00 [pmc-release]
AID - 27/5/1097 [pii]
AID - 3184698 [pii]
AID - 10.1523/JNEUROSCI.3590-06.2007 [doi]
PST - ppublish
SO  - J Neurosci. 2007 Jan 31;27(5):1097-105. doi: 10.1523/JNEUROSCI.3590-06.2007.