PMID- 17267762
OWN - NLM
STAT- MEDLINE
DCOM- 20070604
LR  - 20191210
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 81
IP  - 4
DP  - 2007 Apr
TI  - Artificially generated dendritic cells misdirect antiviral immune responses.
PG  - 952-6
AB  - Dendritic cells (DCs) are critical to the outcome of many viral infections. 
      Questions still remain as to the relevance of artificially generated DCs in 
      models of in vivo immune responses. We compared different DC generation pathways, 
      in terms of phenotypic expression, cytokine production, apoptosis, and T cell 
      proliferation, following viral infection. Direct viral infection of monocytes or 
      monocytes cultured with supernatants from virally infected lung epithelial cells 
      (A549 DCs) induce distinct DC subsets compared with viral infection of 
      artificially generated IL-4 DCs and IFN-DCs. These virally infected DC subsets 
      stimulated different cytokine secretion profiles and displayed contrasting 
      sensitivities to viral-induced apoptosis. It is most interesting that we observed 
      marked differences in the proliferation of purified CD3+ T cells from the virally 
      infected DC subsets. In conclusion, artificially generated DCs skew immune 
      responses to viral infections, and direct viral infection of monocytes and DCs, 
      generated from monocytes cultured with supernatants from infected epithelial 
      cells, appears to be a more relevant pathway of producing DCs, which mimic those 
      generated in vivo.
FAU - Noone, Cariosa
AU  - Noone C
AD  - Viral Immunology Group, School of Biotechnology, Dublin City University, Dublin 
      9, Ireland.
FAU - Manahan, Ellen
AU  - Manahan E
FAU - Newman, Robert
AU  - Newman R
FAU - Johnson, Patricia
AU  - Johnson P
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20070131
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antigens, Differentiation)
RN  - 0 (CD3 Complex)
RN  - 0 (Cytokines)
RN  - 0 (Interferon-alpha)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Antigens, Differentiation/*metabolism
MH  - Apoptosis
MH  - CD3 Complex/metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/virology
MH  - Humans
MH  - *Immunity, Cellular
MH  - Interferon-alpha/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Monocytes/immunology/physiology/*virology
MH  - Respirovirus Infections/immunology
MH  - Tissue Culture Techniques/*methods
EDAT- 2007/02/03 09:00
MHDA- 2007/06/05 09:00
CRDT- 2007/02/03 09:00
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/06/05 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - jlb.1006615 [pii]
AID - 10.1189/jlb.1006615 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2007 Apr;81(4):952-6. doi: 10.1189/jlb.1006615. Epub 2007 Jan 31.