PMID- 17268177
OWN - NLM
STAT- MEDLINE
DCOM- 20070402
LR  - 20151119
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 116
IP  - 1-2
DP  - 2007
TI  - Inverted duplication pattern in anaphase bridges confirms the 
      breakage-fusion-bridge (BFB) cycle model for 11q13 amplification.
PG  - 46-52
AB  - The homogeneously staining region (hsr) involving chromosome band 11q13 includes 
      amplified genes from this chromosome segment and carries a relatively poor 
      prognosis in oral squamous cell carcinomas (OSCC), with shorter time to 
      recurrence and reduced overall survival. We previously identified an inverted 
      duplication pattern of genes within the 11q13 hsr in OSCC cells, supporting a 
      breakage-fusion-bridge (BFB) cycle model for gene amplification. To validate our 
      hypothesis that 11q13 gene amplification in OSCC occurs via BFB cycles, we 
      carried out fluorescence in situ hybridization (FISH) using probes for band 11q13 
      on 29 OSCC cell lines. We demonstrate that all OSCC cell lines with 11q13 
      amplification express a significantly higher frequency of anaphase bridges 
      containing 11q13 sequences compared to cell lines without amplification, 
      providing further experimental evidence that 11q13 gene amplification in OSCC 
      cells occurs via BFB cycles. Elucidation of mechanisms responsible for initiating 
      and promoting gene amplification provides opportunities to identify new 
      biomarkers to aid in the diagnosis and prognosis of oral cancer, and may be 
      useful for developing novel therapeutic strategies for patients with OSCC.
CI  - Copyright 2007 S. Karger AG, Basel.
FAU - Reshmi, S C
AU  - Reshmi SC
AD  - Department of Human Genetics, University of Pittsburgh Graduate School of Public 
      Health, Pittsburgh, PA 15261, USA.
FAU - Roychoudhury, S
AU  - Roychoudhury S
FAU - Yu, Z
AU  - Yu Z
FAU - Feingold, E
AU  - Feingold E
FAU - Potter, D
AU  - Potter D
FAU - Saunders, W S
AU  - Saunders WS
FAU - Gollin, S M
AU  - Gollin SM
LA  - eng
GR  - D43TW06180/TW/FIC NIH HHS/United States
GR  - P30CA47904/CA/NCI NIH HHS/United States
GR  - R01DE14729/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (Biomarkers)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - *Anaphase
MH  - Biomarkers/chemistry
MH  - Carcinoma, Squamous Cell/genetics
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - *Chromosomes, Human, Pair 11
MH  - DNA Damage
MH  - Exons
MH  - *Gene Duplication
MH  - Gene Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Models, Genetic
MH  - Mouth Neoplasms/genetics
MH  - Oligonucleotide Probes
EDAT- 2007/02/03 09:00
MHDA- 2007/04/03 09:00
CRDT- 2007/02/03 09:00
PHST- 2006/05/05 00:00 [received]
PHST- 2006/07/03 00:00 [accepted]
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/04/03 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - 000097425 [pii]
AID - 10.1159/000097425 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2007;116(1-2):46-52. doi: 10.1159/000097425.