PMID- 17268192
OWN - NLM
STAT- MEDLINE
DCOM- 20070402
LR  - 20070201
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 116
IP  - 1-2
DP  - 2007
TI  - Reassignment of chicken W chromosome sequences to the Z chromosome by 
      fluorescence in situ hybridization (FISH).
PG  - 132-4
AB  - There is much interest in the gene content of the small heterochromatic W 
      chromosome of the chicken, on the supposition that it may contain sex-determining 
      genes. A considerable region in the chicken genome has been assigned to the W 
      chromosome on the basis of its repetitive sequences. Using fluorescent in situ 
      hybridization (FISH) we localized five Bacterial Artificial Chromosomes (BACs) 
      onto female chicken metaphase spreads. We physically mapped these BACs to the Z 
      chromosome. The chicken genome database, however, assigned all five BACs to the W 
      chromosome. Our results demonstrate that the 17 genes on these BACs are 
      Z-specific, and points to the inadequacy of assigning regions of the genome based 
      exclusively on repetitive sequences.
CI  - Copyright 2007 S. Karger AG, Basel.
FAU - Stiglec, R
AU  - Stiglec R
AD  - Comparative Genomics Group, Research School of Biological Sciences, The 
      Australian National University, Canberra, Australia. rami.stiglec@anu.edu.au
FAU - Ezaz, T
AU  - Ezaz T
FAU - Graves, J A M
AU  - Graves JA
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Chromosome Mapping
MH  - *Chromosomes
MH  - Chromosomes, Artificial, Bacterial
MH  - DNA Primers/chemistry
MH  - Databases, Genetic
MH  - Female
MH  - Genome
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Hybridization
MH  - Physical Chromosome Mapping
MH  - Sex Chromosomes
EDAT- 2007/02/03 09:00
MHDA- 2007/04/03 09:00
CRDT- 2007/02/03 09:00
PHST- 2006/04/07 00:00 [received]
PHST- 2006/05/26 00:00 [accepted]
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/04/03 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - 000097432 [pii]
AID - 10.1159/000097432 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2007;116(1-2):132-4. doi: 10.1159/000097432.