PMID- 17268847
OWN - NLM
STAT- MEDLINE
DCOM- 20070801
LR  - 20181113
IS  - 0364-3190 (Print)
IS  - 0364-3190 (Linking)
VI  - 32
IP  - 3
DP  - 2007 Mar
TI  - Nicotine inhibits bFGF-induced neurite outgrowth through suppression of NO 
      synthesis in H19-7 cells.
PG  - 481-8
AB  - NO (Nitric oxide) has been known as a biological signaling molecule that can 
      function as a beneficial agent in physiologically essential functions such as 
      differentiation or neurotransmission. In this study, we elucidated how nicotine 
      inhibits neuronal differentiation induced by the basic fibroblast growth factor 
      (bFGF) in hippocampal cell line, H19-7 cells, because nicotine is one of the key 
      neuroregulatory components. Treatment of H19-7 cells with bFGF increased NO 
      production through upregulated iNOS/ nNOS expression, and also increased 
      expressions of neuronal markers such as brain-derived neurotrophic factor (BDNF), 
      neurotrophin-3 (NT3) and Neuro-D. Pretreatment of the cells with nicotine 
      decreased iNOS promoter activity as well as iNOS/nNOS expression induced by bFGF, 
      resulting in decreased NO production. Nicotine also suppressed expressions of 
      BDNF, NT3 and Neuro-D, resulting in decreased bFGF-induced neurite outgrowth. 
      These results indicate that nicotine inhibits bFGF-induced neuronal 
      differentiation in H19-7 cells through inhibition of NO formation by suppressing 
      iNOS/nNOS expressions.
FAU - Shin-young, Park
AU  - Shin-young P
AD  - Department of Biochemistry and Molecular Biology, Hanyang University, 17 
      Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea.
FAU - Koh, Young Jae
AU  - Koh YJ
FAU - Cho, Ju Hwan
AU  - Cho JH
FAU - Oh, Doo-Yi
AU  - Oh DY
FAU - Shin, Su-A
AU  - Shin SA
FAU - Lee, Ki-Sung
AU  - Lee KS
FAU - Lee, Ha-Baik
AU  - Lee HB
FAU - Han, Joong-Soo
AU  - Han JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (DNA Primers)
RN  - 0 (Nerve Tissue Proteins)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 6M3C89ZY6R (Nicotine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - DNA Primers
MH  - Fibroblast Growth Factor 2/*antagonists & inhibitors/*pharmacology
MH  - Hippocampus/drug effects/physiology
MH  - Nerve Tissue Proteins/genetics
MH  - Neurites/drug effects/*physiology
MH  - Nicotine/*pharmacology
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase Type II/genetics
MH  - Nitric Oxide Synthase Type III/genetics
MH  - Polymerase Chain Reaction
MH  - Rats
EDAT- 2007/02/03 09:00
MHDA- 2007/08/02 09:00
CRDT- 2007/02/03 09:00
PHST- 2006/10/16 00:00 [received]
PHST- 2006/12/12 00:00 [accepted]
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/08/02 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - 10.1007/s11064-006-9256-y [doi]
PST - ppublish
SO  - Neurochem Res. 2007 Mar;32(3):481-8. doi: 10.1007/s11064-006-9256-y.