PMID- 17269440
OWN - NLM
STAT- MEDLINE
DCOM- 20070618
LR  - 20190608
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 15
IP  - 8-9
DP  - 2006
TI  - Transplantation of bioreactor-produced neural stem cells into the rodent brain.
PG  - 689-97
AB  - The development of new cell replacement strategies using neural stem cells (NSC) 
      may provide an alternative and unlimited cell source for clinical neural 
      transplantation in neurodegenerative diseases such as Parkinson's and 
      Huntington's disease. The clinical application of neural transplantation using 
      NSC will therefore depend upon the availability of clinical grade NSC that are 
      generated in unlimited quantities in a standardized manner. In order to 
      investigate the utility of NSC in clinical neural transplantation, 
      undifferentiated murine NSC were first expanded for an extended period of time in 
      suspension bioreactors containing a serum-free medium. Following expansion in 
      suspension bioreactors, NSC were still able to differentiate in vitro into both 
      astrocytes and neurons after exposure to brain-derived neurotrophic factor 
      (BDNF), suggesting that bioreactor expansion does not alter cell lineage 
      potentiality. Undifferentiated bioreactor-expanded NSC were then transplanted 
      into the rodent striatum. Immunohistochemical examination revealed 
      undifferentiated bioreactor-expanded NSC survived transplantation for up to 8 
      weeks and expressed the astrocytic immunohistochemical marker glial fibrillary 
      acidic protein (GFAP), suggesting that the host striatal environment influences 
      NSC cell fate upon transplantation. Moreover, no tumor formation was observed 
      within the graft site, indicating that NSC expanded in suspension bioreactors for 
      an extended period of time are a safe source of tissue for transplantation. 
      Future studies should focus on predifferentiating NSC towards specific neuronal 
      phenotypes prior to transplantation in order to restore behavioral function in 
      rodent models of neurodegenerative disease.
FAU - McLeod, M
AU  - McLeod M
AD  - Cell Restoration Laboratory, Brain Repair Centre, Dalhousie University, Halifax, 
      Nova Scotia, Canada. marcus_c_mcleod@hotmail.com
FAU - Hong, M
AU  - Hong M
FAU - Sen, A
AU  - Sen A
FAU - Sadi, D
AU  - Sadi D
FAU - Ulalia, R
AU  - Ulalia R
FAU - Behie, L A
AU  - Behie LA
FAU - Mendez, I
AU  - Mendez I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Animals
MH  - *Bioreactors
MH  - Brain/*surgery
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Survival
MH  - Female
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - Immunohistochemistry
MH  - Ki-67 Antigen/analysis
MH  - Mice
MH  - Microscopy, Confocal
MH  - Neurons/chemistry/cytology/*transplantation
MH  - Rats
MH  - Rats, Wistar
MH  - Stem Cell Transplantation/*methods
MH  - Stem Cells/chemistry/cytology
MH  - Time Factors
EDAT- 2007/02/03 09:00
MHDA- 2007/06/19 09:00
CRDT- 2007/02/03 09:00
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/06/19 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - 10.3727/000000006783464426 [doi]
PST - ppublish
SO  - Cell Transplant. 2006;15(8-9):689-97. doi: 10.3727/000000006783464426.