PMID- 17269964
OWN - NLM
STAT- MEDLINE
DCOM- 20070614
LR  - 20111117
IS  - 0011-9059 (Print)
IS  - 0011-9059 (Linking)
VI  - 46
IP  - 2
DP  - 2007 Feb
TI  - Cross-reactivity of Anisakis simplex: possible role of Ani s 2 and Ani s 3.
PG  - 146-50
AB  - BACKGROUND: Anisakis simplex is a fish parasite that can cause allergy in humans. 
      The multiple cross-reactivities of this nematode make diagnostic tests for 
      allergy unreliable, because of frequent false-positive results. To date, only 
      four allergens of A. simplex have been characterized, but their role in 
      cross-reactivity is largely unknown. METHODS: We performed an amino acid sequence 
      homology analysis, according to the Food and Agriculture Organization/World 
      Health Organization (FAO/WHO) criteria, to identify allergens likely to be 
      cross-reactive with Ani s 2 (paramyosin) and Ani s 3 (tropomyosin) of A. simplex. 
      Next, within segments shared by Ani s 2 or Ani s 3 and homologous proteins, we 
      searched for the occurrence of binding motifs of human leukocyte antigen (HLA) 
      alleles (DRB1*1502, DQB1*0601, or DRB1*0404) known to be genetic risk factors for 
      the development of allergy to A. simplex. RESULTS: We identified a number of 
      proteins belonging to various organisms, which are significantly similar to (and 
      therefore are likely to cross-react with) Ani s 2 and Ani s 3. The presence of 
      binding motifs of HLA DRB1*0404 in Ani s 2, Ani s 3, and homolog proteins also 
      supports our hypothesis. CONCLUSIONS: Our results provide a possible molecular 
      explanation of some cross-reactivities reported for A. simplex, and suggest 
      additional ones.
FAU - Guarneri, Fabrizio
AU  - Guarneri F
AD  - Istituto di Dermatologia, Policlinico Gaetano Martino, Universita di Messina, 
      Messina, Italy. f.guarneri@tiscali.it
FAU - Guarneri, Claudio
AU  - Guarneri C
FAU - Benvenga, Salvatore
AU  - Benvenga S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Dermatol
JT  - International journal of dermatology
JID - 0243704
RN  - 0 (Allergens)
RN  - 0 (Antigens, Helminth)
RN  - 0 (HLA DRB1*04:04 antigen)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (Helminth Proteins)
RN  - 0 (Tropomyosin)
SB  - IM
CIN - Int J Dermatol. 2007 Feb;46(2):224-5. PMID: 17269984
MH  - Allergens/genetics/*immunology
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anisakis/*genetics/*immunology
MH  - Antigens, Helminth/genetics/*immunology
MH  - Cross Reactions
MH  - False Positive Reactions
MH  - HLA-DQ Antigens
MH  - HLA-DQ beta-Chains
MH  - HLA-DR Antigens
MH  - HLA-DRB1 Chains
MH  - Helminth Proteins/genetics/*immunology
MH  - Humans
MH  - Hypersensitivity/diagnosis
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - Tropomyosin/genetics/*immunology
EDAT- 2007/02/03 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/02/03 09:00
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - IJD3091 [pii]
AID - 10.1111/j.1365-4632.2006.03091.x [doi]
PST - ppublish
SO  - Int J Dermatol. 2007 Feb;46(2):146-50. doi: 10.1111/j.1365-4632.2006.03091.x.