PMID- 17270231
OWN - NLM
STAT- MEDLINE
DCOM- 20070712
LR  - 20181113
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 362
IP  - 2
DP  - 2007 Jun 5
TI  - Isolation and characterization of mouse-human microcell hybrid cell clones 
      permissive for infectious HIV particle release.
PG  - 283-93
AB  - Mouse cells are non-permissive to human immunodeficiency virus type 1 (HIV) in 
      that there is a pronounced post-integration block to viral replication. We have 
      recently demonstrated that mouse-human somatic cell hybrids that contain human 
      chromosome 2 increase both HIV Capsid (CA) production and infectious virus 
      release. Here we report on the isolation of three mouse-human microcell hybrids 
      (MCHs) that behave similarly, starting from a pool of 500 MCH clones. Release of 
      virus was specific to HIV and cell revertants that no longer contained any human 
      chromosome fragments did not release CA or infectious virus. Two of the three 
      cell clones were identical as judged by PCR STS content and fluorescence in situ 
      hybridization (FISH) and contained a single 2-12 human chromosome chimera. The 
      third cell clone only contained human chromosome 12, as determined by PCR, FISH, 
      and microarray analyses. There were no consistent differences in Gag protein and 
      spliced/unspliced viral RNA levels between mouse cell lines. CMV promoter-driven, 
      codon-optimized gag-pol had no effect on infectious HIV release from these mouse 
      cells, despite allowing Gag targeting and increasing CA production. These 
      permissive mouse-human MCHs and their corresponding non-permissive revertants may 
      prove useful for mechanistic studies and also for identifying the responsible 
      gene(s) or factor(s) involved in the production of HIV.
FAU - Coskun, Ayse K
AU  - Coskun AK
AD  - Baylor College of Medicine, Department of Molecular Virology and Microbiology, 
      One Baylor Plaza, Houston, TX 77030, USA.
FAU - van Maanen, Marc
AU  - van Maanen M
FAU - Janka, David
AU  - Janka D
FAU - Stockton, David
AU  - Stockton D
FAU - Stankiewicz, Pawel
AU  - Stankiewicz P
FAU - Yatsenko, Svetlana
AU  - Yatsenko S
FAU - Sutton, Richard E
AU  - Sutton RE
LA  - eng
GR  - R21 AI057087-02/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070131
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Codon)
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, gag)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Viral)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
RN  - 0 (gag protein p1, Human immunodeficiency virus)
RN  - 0 (p55 gag precursor protein, Human immunodeficiency virus 1)
SB  - IM
EIN - Virology. 2007 Sep 1;365(2):473. Stankiewicsz, Pawel [corrected to Stankiewicz, 
      Pawel]
MH  - Animals
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 2
MH  - Codon
MH  - DNA, Viral/analysis
MH  - Gene Products, gag/analysis/biosynthesis
MH  - HIV Core Protein p24/biosynthesis
MH  - HIV-1/*growth & development
MH  - Humans
MH  - Hybrid Cells/*virology
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Microarray Analysis
MH  - Polymerase Chain Reaction
MH  - Protein Precursors/analysis
MH  - RNA, Viral/analysis/genetics
MH  - gag Gene Products, Human Immunodeficiency Virus
PMC - PMC1987707
MID - NIHMS23985
EDAT- 2007/02/03 09:00
MHDA- 2007/07/13 09:00
PMCR- 2007/09/20
CRDT- 2007/02/03 09:00
PHST- 2006/08/26 00:00 [received]
PHST- 2006/09/22 00:00 [revised]
PHST- 2006/12/18 00:00 [accepted]
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/07/13 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
PHST- 2007/09/20 00:00 [pmc-release]
AID - S0042-6822(06)00899-3 [pii]
AID - 10.1016/j.virol.2006.12.015 [doi]
PST - ppublish
SO  - Virology. 2007 Jun 5;362(2):283-93. doi: 10.1016/j.virol.2006.12.015. Epub 2007 
      Jan 31.