PMID- 17272926
OWN - NLM
STAT- MEDLINE
DCOM- 20071019
LR  - 20211203
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 53
IP  - 3
DP  - 2007 Jun
TI  - The effect of estrogen on phosphorylation of prolactin in the mouse pituitary 
      gland.
PG  - 515-23
AB  - Several studies have indicated that prolactin (PRL) assumes oligomeric, 
      proteolytically cleaved, phosphorylated and glycosylated forms. Phosphorylated 
      PRL (PPRL) is considered to be the most important posttranslationally modified 
      form in the rat. In the present study, we examined whether or not PRL is present 
      in the mouse pituitary gland in the phosphorylated form. Mouse pituitary PRL was 
      digested with acid phosphatase, resolved by two-dimensional gel electrophoresis, 
      stained with Coomassie brilliant blue, and then immunoblotted against the 
      anti-PRL, anti-phosphoserine and anti-phosphothreonine antibodies. We also 
      examined whether PRL is phosphorylated by protein kinases and semi-quantified the 
      ratios of PPRL to PRL in the pituitary gland. The results indicated that three 
      types of PRL are present in the pituitary glands of both male and female mice. 
      One was non-phosphorylated (isoform 1), and the other two were immunoreactive to 
      anti-phosphoserine (isoform 2) and/or anti-phosphothreonine (isoform 3) 
      antibodies. The ratio between isoforms 2 and 1 of the 30-day-old female mice was 
      higher than that of the 20-day-old female mice. However, the ratios among the 
      three isoforms in the male pituitary glands did not differ with age. The ratio of 
      PPRL to isoform 1 was obviously reduced after ovariectomy (OVX), and it recovered 
      with estrogen replacement. These results suggest that estrogen influences PRL 
      phosphorylation in female mice.
FAU - Horiguchi, Kotaro
AU  - Horiguchi K
AD  - Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of 
      Agriculture, Meiji University, Japan.
FAU - Naito, Jun-ichiro
AU  - Naito J
FAU - Ishida, Michiyo
AU  - Ishida M
FAU - Harigaya, Toshio
AU  - Harigaya T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070202
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 0 (Estrogens)
RN  - 0 (Protein Isoforms)
RN  - 9002-62-4 (Prolactin)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Densitometry
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Estrogens/*metabolism
MH  - Female
MH  - Immunoblotting
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Phosphorylation
MH  - Pituitary Gland/*metabolism
MH  - Pregnancy
MH  - Prolactin/*metabolism
MH  - Protein Isoforms/metabolism
MH  - Protein Serine-Threonine Kinases/*metabolism
EDAT- 2007/02/03 09:00
MHDA- 2007/10/20 09:00
CRDT- 2007/02/03 09:00
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/10/20 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - JST.JSTAGE/jrd/18119 [pii]
AID - 10.1262/jrd.18119 [doi]
PST - ppublish
SO  - J Reprod Dev. 2007 Jun;53(3):515-23. doi: 10.1262/jrd.18119. Epub 2007 Feb 2.