PMID- 17273866
OWN - NLM
STAT- MEDLINE
DCOM- 20070806
LR  - 20220408
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 454
IP  - 2
DP  - 2007 May
TI  - Calcineurin A and CaMKIV transactivate PGC-1alpha promoter, but differentially 
      regulate cytochrome c promoter in rat skeletal muscle.
PG  - 297-305
AB  - In skeletal muscle, slow-twitch fibers are highly dependent on mitochondrial 
      oxidative metabolism suggesting the existence of common regulatory pathways in 
      the control of slow muscle-specific protein expression and mitochondrial 
      biogenesis. In this study, we determined whether peroxisome 
      proliferator-activated receptor gamma co-activator-1alpha (PGC-1alpha) could 
      transactivate promoters of nuclear-encoded mitochondrial protein (cytochrome c) 
      and muscle-specific proteins (fast troponin I, MyoD). We also investigated if 
      calcineurin A (CnA) and calcium/calmodulin kinase IV (CaMKIV) were involved in 
      the regulation of PGC-1alpha and cytochrome c promoter. For this purpose, we took 
      advantage of the gene electrotransfer technique, which allows acute expression of 
      a gene of interest. Electrotransfer of a PGC-1alpha expression vector into rat 
      Tibialis anterior muscle induced a strong transactivation of cytochrome c 
      promoter (P < 0.001) independent of nuclear respiratory factor 1. PGC-1alpha gene 
      electrotransfer did not transactivate fast troponin I promoter, whereas it did 
      transactivate MyoD promoter (P < 0.05). Finally, whereas electrotransfers of CnA 
      or CaMKIV expression vectors transactivated PGC-1alpha promoter (P < 0.001), gene 
      electrotransfer of CaMKIV was only able to transactivate cytochrome c promoter. 
      Taken together, these data suggest that CnA triggers PGC-1alpha promoter 
      transactivation to drive the expression of non-mitochondrial proteins.
FAU - Guerfali, Ibtissem
AU  - Guerfali I
AD  - Unite Physiologie et Physiopathologie de l'Exercice et Handicap EA 3062, Faculte 
      de Medecine, Universite Jean Monnet, 15 rue Ambroise Pare, 42023 Saint-Etienne 
      Cedex 2, France.
FAU - Manissolle, Chloe
AU  - Manissolle C
FAU - Durieux, Anne-Cecile
AU  - Durieux AC
FAU - Bonnefoy, Regis
AU  - Bonnefoy R
FAU - Bartegi, Aghleb
AU  - Bartegi A
FAU - Freyssenet, Damien
AU  - Freyssenet D
LA  - eng
PT  - Journal Article
DEP - 20070202
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (MyoD Protein)
RN  - 0 (Nuclear Respiratory Factor 1)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Ppargc1a protein, rat)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Troponin I)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 4)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.17 (Camk4 protein, rat)
RN  - EC 3.1.3.16 (Calcineurin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calcineurin/genetics/*metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 4
MH  - Calcium-Calmodulin-Dependent Protein Kinases/genetics/*metabolism
MH  - Cytochromes c/*genetics/metabolism
MH  - Gene Deletion
MH  - Luciferases/genetics/metabolism
MH  - Male
MH  - Muscle, Skeletal/*metabolism
MH  - MyoD Protein/genetics/metabolism
MH  - Nuclear Respiratory Factor 1/genetics/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - Promoter Regions, Genetic/*genetics
MH  - RNA-Binding Proteins/*genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Transcription Factors/*genetics/metabolism
MH  - Transcriptional Activation
MH  - Transfection
MH  - Troponin I/genetics/metabolism
EDAT- 2007/02/03 09:00
MHDA- 2007/08/07 09:00
CRDT- 2007/02/03 09:00
PHST- 2006/10/17 00:00 [received]
PHST- 2007/01/04 00:00 [accepted]
PHST- 2006/12/21 00:00 [revised]
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2007/08/07 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - 10.1007/s00424-007-0206-6 [doi]
PST - ppublish
SO  - Pflugers Arch. 2007 May;454(2):297-305. doi: 10.1007/s00424-007-0206-6. Epub 2007 
      Feb 2.