PMID- 17273899
OWN - NLM
STAT- MEDLINE
DCOM- 20080521
LR  - 20071024
IS  - 0016-6707 (Print)
IS  - 0016-6707 (Linking)
VI  - 131
IP  - 3
DP  - 2007 Nov
TI  - Characterization of Japanese flounder karyotype by chromosome bandings and 
      fluorescence in situ hybridization with DNA markers.
PG  - 267-74
AB  - The chromosomes of Japanese flounder, Paralichthys olivaceus, were examined by 
      conventional differential staining methods including G-, Q-, C-, silver (Ag)-, 
      fluorochrome, and replication R-bandings and by fluorescence in situ 
      hybridization (FISH) with 5S and 18S rDNAs and telomeric DNA as probes. 
      Replication R-banding substantially made it possible to identify 24 homologous 
      pairs by their RBG-banding pattern and relative length. Both rDNA loci were 
      mapped to chromosome 1, where 5S and 18S rDNA loci were located at the 
      centromeric region and secondary constriction, respectively. C-banding revealed 
      that both rDNA loci were heterochromatic, and 18S rDNA loci were positive for 
      chromomycin A(3) but negative for 4',6-diamidino-2-phenylindole (DAPI) staining. 
      Telomeric FISH signals were observed at all chromosome ends and at the 
      interstitial region of some chromosomes. The observed results were discussed in 
      relation to the karyotype evolution in the order Pleuronectiformes.
FAU - Fujiwara, Atushi
AU  - Fujiwara A
AD  - Inland Station, Fisheries Research Agency, National Research Institute of 
      Aquaculture, Tamaki Mie 519-0423, Japan. jiwara@fra.affrc.go.jp
FAU - Fujiwara, Mika
AU  - Fujiwara M
FAU - Nishida-Umehara, Chizuko
AU  - Nishida-Umehara C
FAU - Abe, Syuiti
AU  - Abe S
FAU - Masaoka, Tetsuji
AU  - Masaoka T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070202
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - Cytogenetic Analysis/*methods
MH  - DNA, Ribosomal
MH  - *Evolution, Molecular
MH  - Flounder/*genetics
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/*methods
MH  - Staining and Labeling
MH  - Telomere
EDAT- 2007/02/03 09:00
MHDA- 2008/05/22 09:00
CRDT- 2007/02/03 09:00
PHST- 2006/06/23 00:00 [received]
PHST- 2006/12/23 00:00 [accepted]
PHST- 2007/02/03 09:00 [pubmed]
PHST- 2008/05/22 09:00 [medline]
PHST- 2007/02/03 09:00 [entrez]
AID - 10.1007/s10709-006-9136-z [doi]
PST - ppublish
SO  - Genetica. 2007 Nov;131(3):267-74. doi: 10.1007/s10709-006-9136-z. Epub 2007 Feb 
      2.