PMID- 17274799
OWN - NLM
STAT- MEDLINE
DCOM- 20070827
LR  - 20181201
IS  - 1398-9219 (Print)
IS  - 1398-9219 (Linking)
VI  - 8
IP  - 3
DP  - 2007 Mar
TI  - The interaction of two tethering factors, p115 and COG complex, is required for 
      Golgi integrity.
PG  - 270-84
AB  - The vesicle-tethering protein p115 functions in endoplasmic reticulum-Golgi 
      trafficking. We explored the function of homologous region 2 (HR2) of the p115 
      head domain that is highly homologous with the yeast counterpart, Uso1p. By 
      expression of p115 mutants in p115 knockdown (KD) cells, we found that deletion 
      of HR2 caused an irregular assembly of the Golgi, which consisted of a cluster of 
      mini-stacked Golgi fragments, and gathered around microtubule-organizing center 
      in a microtubule-dependent manner. Protein interaction analyses revealed that 
      p115 HR2 interacted with Cog2, a subunit of the conserved oligomeric Golgi (COG) 
      complex that is known another putative cis-Golgi vesicle-tethering factor. The 
      interaction between p115 and Cog2 was found to be essential for Golgi ribbon 
      reformation after the disruption of the ribbon by p115 KD or brefeldin A 
      treatment and recovery by re-expression of p115 or drug wash out, respectively. 
      The interaction occurred only in interphase cells and not in mitotic cells. These 
      results strongly suggested that p115 plays an important role in the biogenesis 
      and maintenance of the Golgi by interacting with the COG complex on the cis-Golgi 
      in vesicular trafficking.
FAU - Sohda, Miwa
AU  - Sohda M
AD  - Department of Cell Biology, Fukuoka University School of Medicine, Jonan-ku, 
      Fukuoka 814-0180, Japan.
FAU - Misumi, Yoshio
AU  - Misumi Y
FAU - Yoshimura, Shin-Ichiro
AU  - Yoshimura S
FAU - Nakamura, Nobuhiro
AU  - Nakamura N
FAU - Fusano, Takami
AU  - Fusano T
FAU - Ogata, Shigenori
AU  - Ogata S
FAU - Sakisaka, Shotaro
AU  - Sakisaka S
FAU - Ikehara, Yukio
AU  - Ikehara Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070126
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Golgi Matrix Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (SNARE Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 160124-07-2 (COG2 protein, human)
RN  - 162079-20-1 (vesicular transport factor p115)
RN  - 20350-15-6 (Brefeldin A)
SB  - IM
MH  - Adaptor Proteins, Vesicular Transport/*metabolism
MH  - Brefeldin A/pharmacology
MH  - Endoplasmic Reticulum/metabolism
MH  - Golgi Apparatus/*metabolism
MH  - Golgi Matrix Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mutation
MH  - Protein Interaction Mapping
MH  - Protein Structure, Tertiary
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Protein Transport
MH  - SNARE Proteins/metabolism
MH  - Vesicular Transport Proteins/genetics/*metabolism
EDAT- 2007/02/06 09:00
MHDA- 2007/08/28 09:00
CRDT- 2007/02/06 09:00
PHST- 2007/02/06 09:00 [pubmed]
PHST- 2007/08/28 09:00 [medline]
PHST- 2007/02/06 09:00 [entrez]
AID - TRA530 [pii]
AID - 10.1111/j.1600-0854.2006.00530.x [doi]
PST - ppublish
SO  - Traffic. 2007 Mar;8(3):270-84. doi: 10.1111/j.1600-0854.2006.00530.x. Epub 2007 
      Jan 26.