PMID- 17275194 OWN - NLM STAT- MEDLINE DCOM- 20070626 LR - 20151119 IS - 0306-4522 (Print) IS - 0306-4522 (Linking) VI - 145 IP - 2 DP - 2007 Mar 16 TI - Tinnitus behavior and hearing function correlate with the reciprocal expression patterns of BDNF and Arg3.1/arc in auditory neurons following acoustic trauma. PG - 715-26 AB - The molecular changes following sensory trauma and the subsequent response of the CNS are poorly understood. We focused on finding a molecular tool for monitoring the features of excitability which occur following acoustic trauma to the auditory system. Of particular interest are genes that alter their expression pattern during activity-induced changes in synaptic efficacy and plasticity. The expression of brain-derived neurotrophic factor (BDNF), the activity-dependent cytoskeletal protein (Arg3.1/arc), and the immediate early gene c-Fos were monitored in the peripheral and central auditory system hours and days following a traumatic acoustic stimulus that induced not only hearing loss but also phantom auditory perception (tinnitus), as shown in rodent animal behavior models. A reciprocal responsiveness of activity-dependent genes became evident between the periphery and the primary auditory cortex (AI): as c-Fos and BDNF exon IV expression was increased in spiral ganglion neurons, Arg3.1/arc and (later on) BDNF exon IV expression was reduced in AI. In line with studies indicating increased spontaneous spike activity at the level of the inferior colliculus (IC), an increase in BDNF and GABA-positive neurons was seen in the IC. The data clearly indicate the usefulness of Arg3.1/arc and BDNF for monitoring trauma-induced activity changes and the associated putative plasticity responses in the auditory system. FAU - Tan, J AU - Tan J AD - University of Tubingen, Department of Otorhinolaryngology, Hearing Research Center Tubingen, Molecular Neurobiology, Elfriede-Aulhorn-Strasse 5, 72076 Tubingen, Germany. FAU - Ruttiger, L AU - Ruttiger L FAU - Panford-Walsh, R AU - Panford-Walsh R FAU - Singer, W AU - Singer W FAU - Schulze, H AU - Schulze H FAU - Kilian, S B AU - Kilian SB FAU - Hadjab, S AU - Hadjab S FAU - Zimmermann, U AU - Zimmermann U FAU - Kopschall, I AU - Kopschall I FAU - Rohbock, K AU - Rohbock K FAU - Knipper, M AU - Knipper M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070201 PL - United States TA - Neuroscience JT - Neuroscience JID - 7605074 RN - 0 (Biomarkers) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Cytoskeletal Proteins) RN - 0 (Nerve Tissue Proteins) RN - 0 (Proto-Oncogene Proteins c-fos) RN - 0 (activity regulated cytoskeletal-associated protein) SB - IM MH - Action Potentials/physiology MH - Animals MH - Auditory Cortex/cytology/metabolism MH - Auditory Pathways/cytology/metabolism MH - Biomarkers/analysis/metabolism MH - Brain-Derived Neurotrophic Factor/*genetics MH - Cytoskeletal Proteins/*genetics MH - Ear, Inner/injuries/*metabolism/physiopathology MH - Female MH - Gene Expression/physiology MH - Hearing Loss, Noise-Induced/*metabolism/physiopathology MH - Nerve Tissue Proteins/*genetics MH - Neuronal Plasticity/physiology MH - Neurons, Afferent/*metabolism MH - Noise/adverse effects MH - Proto-Oncogene Proteins c-fos/genetics MH - Rats MH - Rats, Wistar MH - Spiral Ganglion/cytology/metabolism MH - Tinnitus/*metabolism/physiopathology MH - Up-Regulation/physiology EDAT- 2007/02/06 09:00 MHDA- 2007/06/27 09:00 CRDT- 2007/02/06 09:00 PHST- 2006/05/03 00:00 [received] PHST- 2006/11/28 00:00 [revised] PHST- 2006/11/30 00:00 [accepted] PHST- 2007/02/06 09:00 [pubmed] PHST- 2007/06/27 09:00 [medline] PHST- 2007/02/06 09:00 [entrez] AID - S0306-4522(06)01667-8 [pii] AID - 10.1016/j.neuroscience.2006.11.067 [doi] PST - ppublish SO - Neuroscience. 2007 Mar 16;145(2):715-26. doi: 10.1016/j.neuroscience.2006.11.067. Epub 2007 Feb 1.