PMID- 1727811
OWN - NLM
STAT- MEDLINE
DCOM- 19920130
LR  - 20181130
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 74
IP  - 1
DP  - 1992 Jan
TI  - Tumor necrosis factor-alpha inhibits human chorionic gonadotropin secretion.
PG  - 130-4
AB  - The placenta is a major source of tumor necrosis factor-alpha (TNF alpha) and is 
      rich in TNF alpha receptors. TNF alpha inhibited hCG secretion by normal 
      chorionic villi at 6 weeks gestation, but chorionic villi contain the 
      heterogenous cell population. To investigate the direct effects of TNF alpha on 
      trophoblasts, the NUC1 choriocarcinoma cell line was used as an in vitro 
      placental cell model. TNF alpha also inhibited hCG secretion by NUC1 cells at 
      concentrations from 1-100 U/mL. TNF alpha at concentrations of 1, 10, and 100 
      U/mL significantly decreased hCG secretion for 24 h to 88%, 81%, and 71% of the 
      control level, respectively. The expression of hCG beta mRNA was also decreased 
      to 23% of the control level after 24 h of TNF alpha treatment (100 U/mL). 
      Inhibition occurred after 12 h of TNF alpha treatment (100 U/mL). Two 
      measurements, trypan blue dye exclusion and 
      3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide reduction, revealed 
      that these inhibitory effects were not due to the cytotoxic activity of TNF alpha 
      on NUC1. In conclusion, TNF alpha reduces hCG secretion in vitro.
FAU - Ohashi, K
AU  - Ohashi K
AD  - Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
FAU - Saji, F
AU  - Saji F
FAU - Kato, M
AU  - Kato M
FAU - Wakimoto, A
AU  - Wakimoto A
FAU - Tanizawa, O
AU  - Tanizawa O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (beta 2-Microglobulin)
RN  - EUY85H477I (thiazolyl blue)
RN  - I2ZWO3LS3M (Trypan Blue)
SB  - IM
MH  - Blotting, Northern
MH  - Cell Division/drug effects
MH  - Chorionic Gonadotropin/*antagonists & inhibitors/genetics/metabolism
MH  - Female
MH  - Humans
MH  - Pregnancy/metabolism
MH  - Pregnancy Trimester, First
MH  - RNA, Messenger/analysis
MH  - Radioimmunoassay
MH  - Tetrazolium Salts
MH  - Thiazoles
MH  - Trophoblasts/metabolism
MH  - Trypan Blue
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - beta 2-Microglobulin/metabolism
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1210/jcem.74.1.1727811 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1992 Jan;74(1):130-4. doi: 10.1210/jcem.74.1.1727811.