PMID- 17278130
OWN - NLM
STAT- MEDLINE
DCOM- 20070501
LR  - 20161122
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 501
IP  - 4
DP  - 2007 Apr 1
TI  - Differential distribution of the MeCP2 splice variants in the postnatal mouse 
      brain.
PG  - 526-42
AB  - Mutations in the gene encoding methyl CpG binding protein 2 (MeCP2) are the 
      primary cause of the neurodevelopmental disorder Rett syndrome (RTT). 
      Mecp2-deficient mice develop a neurological phenotype that recapitulates many of 
      the symptoms of RTT, including postnatal onset of the neurological deficits. 
      MeCP2 has two isoforms, MeCP2e1 and MeCP2e2, with distinct amino termini, which 
      are generated by alternative splicing. We examined the distribution of the Mecp2 
      splice variants in the postnatal mouse brain by in situ hybridization and found 
      regional and age-related differences in transcript abundance. In newborn mice, 
      signals for total Mecp2 and the Mecp2e2 transcripts were widely distributed, with 
      overlapping expression patterns throughout the brain. Expression of the Mecp2e2 
      splice variant became largely restricted to nuclei within the dorsal thalamus 
      (DT) and cortical layer V in juvenile animals, a pattern that was maintained into 
      adulthood. In contrast, the total Mecp2 riboprobe only weakly labeled the DT and 
      cortical layer V in juvenile and adult animals, although it heavily labeled 
      surrounding brain regions, suggesting that Mecp2e1 is the predominant transcript 
      outside the thalamus. Quantitative real-time PCR was used to measure Mecp2e1 and 
      Mecp2e2 abundance in the diencephalon of adult mice, demonstrating significantly 
      more Mecp2e2 in the DT than in the hypothalamus, which is in agreement with the 
      Mecp2e2 in situ hybridization. The differential distribution of the Mecp2e1 and 
      Mecp2e2 transcripts indicates regional and developmental regulation of Mecp2 
      splicing in the postnatal mouse brain.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Dragich, Joanna M
AU  - Dragich JM
AD  - Neuroscience Interdepartmental PhD Program, University of California, Los 
      Angeles, Los Angeles, California 90095-1761, USA.
FAU - Kim, Yong-Hwan
AU  - Kim YH
FAU - Arnold, Arthur P
AU  - Arnold AP
FAU - Schanen, N Carolyn
AU  - Schanen NC
LA  - eng
GR  - F31 NS043072/NS/NINDS NIH HHS/United States
GR  - F31 NS043072-03/NS/NINDS NIH HHS/United States
GR  - R01HD37874/HD/NICHD NIH HHS/United States
GR  - P20RR020173/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Mecp2 protein, mouse)
RN  - 0 (Methyl-CpG-Binding Protein 2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Age Factors
MH  - *Alternative Splicing
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/growth & development/*metabolism
MH  - Brain Mapping
MH  - Female
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - In Situ Hybridization/methods
MH  - Male
MH  - Methyl-CpG-Binding Protein 2/*genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
EDAT- 2007/02/06 09:00
MHDA- 2007/05/02 09:00
CRDT- 2007/02/06 09:00
PHST- 2007/02/06 09:00 [pubmed]
PHST- 2007/05/02 09:00 [medline]
PHST- 2007/02/06 09:00 [entrez]
AID - 10.1002/cne.21264 [doi]
PST - ppublish
SO  - J Comp Neurol. 2007 Apr 1;501(4):526-42. doi: 10.1002/cne.21264.