PMID- 1727819
OWN - NLM
STAT- MEDLINE
DCOM- 19920130
LR  - 20061115
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 74
IP  - 1
DP  - 1992 Jan
TI  - Trophoblast-derived tumor necrosis factor-alpha induces release of human 
      chorionic gonadotropin using interleukin-6 (IL-6) and IL-6-receptor-dependent 
      system in the normal human trophoblasts.
PG  - 184-91
AB  - The titer of tumor necrosis factor-alpha (TNF alpha) secreted by placental blocks 
      was determined by enzyme immunoassay. The source of placental TNF alpha was 
      immunohistochemically demonstrated with monoclonal anti-TNF alpha antibody to be 
      only trophoblasts. Purified trophoblasts produced 174.4 ng/L TNF alpha by 24 h of 
      culture in vitro. To investigate the role of TNF alpha in placental 
      hormonogenesis, purified trophoblasts were stimulated with recombinant TNF alpha 
      (rTNF alpha) to determine the hCG titer by enzyme immunoassay. Trophoblasts 
      stimulated with rTNF alpha released hCG in a dose-dependent fashion with kinetics 
      similar to those of recombinant interleukin-1 (rIL-1)-stimulated trophoblasts. 
      The stimulated trophoblasts released IL-6 before hCG, but failed to show hCG 
      release when pretreated with anti-IL-6 receptor (anti-IL-6-R) monoclonal antibody 
      PM-1. However, the pretreatment of trophoblasts with PM-1 did not interfere with 
      rTNF alpha-induced IL-6 release, ruling out the possibility of a nonspecific 
      toxic effect of PM-1 on trophoblasts. These results suggest that 
      trophoblast-derived TNF alpha induced IL-6 release and then activated the IL-6-R 
      system in trophoblasts to release hCG. Since IL-1 has also been demonstrated to 
      induce similar release of IL-6 and hCG from trophoblasts, the effects of TNF 
      alpha and IL-1 on these trophoblast functions were also examined. Simultaneous 
      stimulation of trophoblasts with rTNF alpha and gamma IL-1 alpha resulted in 
      synergistic enhancement of IL-6 release, subsequently leading to enhanced hCG 
      release. Collectively, trophoblast-derived TNF alpha and IL-1 synergistically 
      regulated the level of IL-6 secreted by trophoblasts, the magnitude of which 
      determined the level of hCG released by activating the IL-6-R system in 
      trophoblasts.
FAU - Li, Y
AU  - Li Y
AD  - Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
FAU - Matsuzaki, N
AU  - Matsuzaki N
FAU - Masuhiro, K
AU  - Masuhiro K
FAU - Kameda, T
AU  - Kameda T
FAU - Taniguchi, T
AU  - Taniguchi T
FAU - Saji, F
AU  - Saji F
FAU - Yone, K
AU  - Yone K
FAU - Tanizawa, O
AU  - Tanizawa O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Chorionic Gonadotropin/antagonists & inhibitors/*metabolism
MH  - Drug Synergism
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Interleukin-6/*pharmacology
MH  - Receptors, Immunologic/immunology/*physiology
MH  - Receptors, Interleukin-6
MH  - Recombinant Proteins
MH  - Reference Values
MH  - Trophoblasts/chemistry/*metabolism
MH  - Tumor Necrosis Factor-alpha/analysis/*pharmacology
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1210/jcem.74.1.1727819 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1992 Jan;74(1):184-91. doi: 10.1210/jcem.74.1.1727819.