PMID- 17280688
OWN - NLM
STAT- MEDLINE
DCOM- 20070329
LR  - 20141120
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 80
IP  - 12
DP  - 2007 Feb 27
TI  - Attenuation of 4-nitroquinoline 1-oxide induced in vitro lipid peroxidation by 
      green tea polyphenols.
PG  - 1080-6
AB  - Lipid peroxidation is believed to play an important role in pathogenesis of 
      diseases. 4-Nitroquiunoline 1-oxide (4-NQO) a potent oral carcinogen, widely used 
      for induction of oral carcinogenesis, was found to induce lipid peroxidation in 
      vivo and in vitro. Green tea contains high content of polyphenols, which are 
      potent antioxidants. Thus green tea polyphenols (GP) can play a protective role 
      in 4-NQO induced in vitro lipid peroxidation. 4-NQO at the concentration of 1.5 
      mM was found to induce lipid peroxidation in 5% liver homogenate in phosphate 
      buffered saline and extent of lipid peroxidation at the different time intervals 
      0, 15, 30 and 45 min where studied by assessing parameters such as hydroxyl 
      radical production (OH), thiobarbituric acid reactants (TBARS), reduced 
      glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). It was found 
      that addition of 4-NQO caused an increase in OH and TBARS level and a decrease in 
      activity of SOD, CAT and the levels of GSH. Simultaneous addition of GP 10 mg/ml 
      significantly decreased lipid peroxidation and increased in antioxidant status. 
      Thus, we conclude that GP, a potent antioxidant, was found to nullify 4-NQO 
      induced lipid peroxidation in vitro and 4-NQO acts initially by causing oxidative 
      stress and leads to carcinogenesis.
FAU - Srinivasan, Periasamy
AU  - Srinivasan P
AD  - Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600 025, 
      Tamilnadu, India.
FAU - Sabitha, Kuruvimalai Ekambaram
AU  - Sabitha KE
FAU - Shyamaladevi, Chennam Srinivasulu
AU  - Shyamaladevi CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061209
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Flavonoids)
RN  - 0 (Lipid Peroxides)
RN  - 0 (Phenols)
RN  - 0 (Polyphenols)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 56-57-5 (4-Nitroquinoline-1-oxide)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - 4-Nitroquinoline-1-oxide/*toxicity
MH  - Animals
MH  - Camellia sinensis/*chemistry
MH  - Catalase/metabolism
MH  - Flavonoids/isolation & purification/*pharmacology
MH  - Glutathione/metabolism
MH  - Hydroxyl Radical/metabolism
MH  - In Vitro Techniques
MH  - Lipid Peroxidation/*drug effects
MH  - Lipid Peroxides/metabolism
MH  - Liver/*drug effects/enzymology/metabolism
MH  - Male
MH  - Phenols/isolation & purification/*pharmacology
MH  - Plant Leaves/chemistry
MH  - Polyphenols
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxide Dismutase/metabolism
EDAT- 2007/02/07 09:00
MHDA- 2007/03/30 09:00
CRDT- 2007/02/07 09:00
PHST- 2006/01/02 00:00 [received]
PHST- 2006/11/08 00:00 [revised]
PHST- 2006/11/14 00:00 [accepted]
PHST- 2007/02/07 09:00 [pubmed]
PHST- 2007/03/30 09:00 [medline]
PHST- 2007/02/07 09:00 [entrez]
AID - S0024-3205(06)00911-8 [pii]
AID - 10.1016/j.lfs.2006.11.051 [doi]
PST - ppublish
SO  - Life Sci. 2007 Feb 27;80(12):1080-6. doi: 10.1016/j.lfs.2006.11.051. Epub 2006 
      Dec 9.