PMID- 17280738
OWN - NLM
STAT- MEDLINE
DCOM- 20070517
LR  - 20070320
IS  - 0171-9335 (Print)
IS  - 0171-9335 (Linking)
VI  - 86
IP  - 4
DP  - 2007 Apr
TI  - Induction of monocyte chemoattractant protein-1 release from A549 cells by 
      agonists of protease-activated receptor-1 and -2.
PG  - 233-42
AB  - Hypersecretion of cytokines and serine proteases has been observed in asthma. 
      However, the influence of proteases and protease-activated receptors (PARs) on 
      monocyte chemoattractant protein-1 (MCP-1) release from airway epithelial cells 
      remains largely unknown. In the present study, A549 cells were challenged with 
      agonists of PARs, and levels of MCP-1 released in the supernatant and mRNA 
      expression were examined by ELISA and real time polymerase chain reaction (PCR), 
      respectively. The results show that thrombin, tryptase, elastase and trypsin 
      induced an up to 6.5-, 1.8-, 1.6-, and 3.1-fold increase in MCP-1 release from 
      A549 cells, respectively, following a 16-h incubation period. The 
      protease-induced secretion of MCP-1 can be abolished by specific protease 
      inhibitors. Agonist peptides of PAR-1 and PAR-2 stimulate MCP-1 secretion up to 
      15- and 12.7-fold, respectively. Real-time PCR showed that MCP-1 mRNA is 
      up-regulated by the serine proteases tested and by agonist peptides of PAR-1 and 
      PAR-2. In conclusion, serine proteases can stimulate MCP-1 release from A549 
      cells possibly through a PARs-related mechanism, suggesting that they are likely 
      to contribute to MCP-1-related airway inflammatory disorders in man.
FAU - Wang, Haiyan
AU  - Wang H
AD  - Allergy and Inflammation Research Institute, The Key Immunopharmacology 
      Laboratory of Guangdong Province, Shantou University Medical College, Shantou 
      515041, China.
FAU - Yi, Tingting
AU  - Yi T
FAU - Zheng, Yanshan
AU  - Zheng Y
FAU - He, Shaoheng
AU  - He S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070205
PL  - Germany
TA  - Eur J Cell Biol
JT  - European journal of cell biology
JID - 7906240
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Oligopeptides)
RN  - 0 (Protease Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, PAR-1)
RN  - 0 (Receptor, PAR-2)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 3.4.21.5 (Thrombin)
RN  - EC 3.4.21.59 (Tryptases)
SB  - IM
MH  - Cell Line, Tumor
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Lung Neoplasms/metabolism/pathology
MH  - Oligopeptides/pharmacology
MH  - Pancreatic Elastase/metabolism
MH  - Protease Inhibitors/pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Receptor, PAR-1/agonists/*metabolism
MH  - Receptor, PAR-2/agonists/*metabolism
MH  - Respiratory Mucosa/*metabolism/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serine Endopeptidases/*metabolism/pharmacology
MH  - Thrombin/*metabolism/pharmacology
MH  - Time Factors
MH  - Trypsin/metabolism
MH  - Tryptases/metabolism
MH  - Up-Regulation
EDAT- 2007/02/07 09:00
MHDA- 2007/05/18 09:00
CRDT- 2007/02/07 09:00
PHST- 2006/06/21 00:00 [received]
PHST- 2006/10/27 00:00 [revised]
PHST- 2006/12/04 00:00 [accepted]
PHST- 2007/02/07 09:00 [pubmed]
PHST- 2007/05/18 09:00 [medline]
PHST- 2007/02/07 09:00 [entrez]
AID - S0171-9335(06)00192-0 [pii]
AID - 10.1016/j.ejcb.2006.12.003 [doi]
PST - ppublish
SO  - Eur J Cell Biol. 2007 Apr;86(4):233-42. doi: 10.1016/j.ejcb.2006.12.003. Epub 
      2007 Feb 5.