PMID- 17283484
OWN - NLM
STAT- MEDLINE
DCOM- 20070320
LR  - 20230124
IS  - 1600-6135 (Print)
IS  - 1600-6135 (Linking)
VI  - 7
IP  - 2
DP  - 2007 Feb
TI  - Mesenchymal stem cells facilitate the induction of mixed hematopoietic chimerism 
      and islet allograft tolerance without GVHD in the rat.
PG  - 336-46
AB  - Induction of hematopoietic chimerism and subsequent donor-specific immune 
      tolerance via bone marrow transplantation is an ideal approach for islet 
      transplantation to treat type-1 diabetes. We examined the potential of 
      mesenchymal stem cells (MSCs) in the induction of chimerism and islet allograft 
      tolerance without the incidence of graft-versus-host disease (GVHD). 
      Streptozotocin-diabetic rats received a conditioning regimen consisting of 
      antilymphocyte serum and 5 Gy total body irradiation, followed by an intraportal 
      co-infusion of allogeneic MSCs, bone marrow cells (BMCs) and islets. Although all 
      the recipients rejected the islets initially, half of them developed stable mixed 
      chimerism and donor-specific immune tolerance, shown by the engraftment of donor 
      skin and second-set islet transplants and acute rejection of a third-party skin. 
      The engraftment of the primary islet allografts with stable chimerism was 
      achieved by the addition of a 2-week peritransplant administration of 
      15-deoxyspergualin (DSG). Without MSCs, none of the recipients treated with DSG 
      developed chimerism or reversal of diabetes. GVHD was not observed in any of the 
      recipients infused with MSCs (0/15), whereas it occurred in 4/11 recipients 
      without MSCs. These results indicate a potential use of MSCs for induction of 
      hematopoietic chimerism and subsequent immune tolerance in clinical islet 
      transplantation.
FAU - Itakura, S
AU  - Itakura S
AD  - Southern California Islet Cell Resource Center, Department of Diabetes, 
      Endocrinology and Metabolism, Duarte, CA, USA. sitakura@coh.org
FAU - Asari, S
AU  - Asari S
FAU - Rawson, J
AU  - Rawson J
FAU - Ito, T
AU  - Ito T
FAU - Todorov, I
AU  - Todorov I
FAU - Liu, C-P
AU  - Liu CP
FAU - Sasaki, N
AU  - Sasaki N
FAU - Kandeel, F
AU  - Kandeel F
FAU - Mullen, Y
AU  - Mullen Y
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Transplant
JT  - American journal of transplantation : official journal of the American Society of 
      Transplantation and the American Society of Transplant Surgeons
JID - 100968638
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*methods
MH  - Cells, Cultured
MH  - *Chimera
MH  - Diabetes Mellitus, Experimental/surgery
MH  - Diabetes Mellitus, Type 1/therapy
MH  - Graft Rejection/immunology/pathology
MH  - Graft vs Host Disease/immunology/*prevention & control
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Immune Tolerance/*immunology
MH  - Immunosuppression Therapy
MH  - Islets of Langerhans Transplantation/*immunology/pathology
MH  - Killer Cells, Natural/immunology/pathology
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred Lew
MH  - Transplantation Tolerance/immunology
MH  - Transplantation, Homologous/immunology
EDAT- 2007/02/07 09:00
MHDA- 2007/03/21 09:00
CRDT- 2007/02/07 09:00
PHST- 2007/02/07 09:00 [pubmed]
PHST- 2007/03/21 09:00 [medline]
PHST- 2007/02/07 09:00 [entrez]
AID - S1600-6135(22)14579-X [pii]
AID - 10.1111/j.1600-6143.2006.01643.x [doi]
PST - ppublish
SO  - Am J Transplant. 2007 Feb;7(2):336-46. doi: 10.1111/j.1600-6143.2006.01643.x.