PMID- 17286314
OWN - NLM
STAT- MEDLINE
DCOM- 20070605
LR  - 20151119
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 27
IP  - 4
DP  - 2007 Apr
TI  - Two-way trafficking of Annexin V positive cells between mother and fetus: 
      determination of apoptosis at delivery.
PG  - 348-51
AB  - OBJECTIVES: The aim of this study was to quantitate apoptosis in maternal 
      circulation and umbilical cord blood (UCB) at delivery. The proportion of fetal 
      cells in maternal blood as well as that of maternal cells in UCB was also 
      determined. MATERIAL AND METHODS: Three milliliters of peripheral blood was 
      collected from nine women during labor. Five women delivered males and four 
      delivered females. Immediately after delivery, 3 mL UCB was collected. Ten 
      microliters was used to quantitate apoptosis by the ethidium bromide assay 
      (EthBr) and from the remaining blood, Annexin V positive cells were isolated by 
      MACS. RESULTS: The Median apoptosis rate in maternal samples was 25% (19-34) and 
      in UCB 20% (16-28). Annexin V positive cells were present in all samples 
      analyzed. As shown by Fluorescence in situ hybridization (FISH) in maternal 
      samples, cells with an XY hybridization pattern were identified in cases with 
      male newborns in a median concentration of 1.7% (1.6-2.1). On the corresponding 
      UCB, a median of 1.2% (0.8-1.6) XX cells were detected. CONCLUSION: The study 
      demonstrates the existence of a bidirectional transfer of fetal and maternal 
      cells under apoptosis across the placenta and provides useful information 
      regarding use of UCB for transplantation.
CI  - Copyright (c) 2007 John Wiley & Sons, Ltd.
FAU - Kolialexi, A
AU  - Kolialexi A
AD  - Department of Medical Genetics, Athens University School of Medicine, Athens, 
      Greece.
FAU - Tsangaris, G Th
AU  - Tsangaris GT
FAU - Anagnostopoulos, A
AU  - Anagnostopoulos A
FAU - Chondros, D
AU  - Chondros D
FAU - Bagiokos, V
AU  - Bagiokos V
FAU - Kitsiou, S
AU  - Kitsiou S
FAU - Kanavakis, E
AU  - Kanavakis E
FAU - Mavrou, A
AU  - Mavrou A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (Annexin A5)
SB  - IM
MH  - Annexin A5
MH  - *Apoptosis
MH  - Blood Cells/*physiology
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Maternal-Fetal Exchange/*physiology
MH  - Pregnancy/*blood
EDAT- 2007/02/09 09:00
MHDA- 2007/06/06 09:00
CRDT- 2007/02/09 09:00
PHST- 2007/02/09 09:00 [pubmed]
PHST- 2007/06/06 09:00 [medline]
PHST- 2007/02/09 09:00 [entrez]
AID - 10.1002/pd.1671 [doi]
PST - ppublish
SO  - Prenat Diagn. 2007 Apr;27(4):348-51. doi: 10.1002/pd.1671.