PMID- 17286902
OWN - NLM
STAT- MEDLINE
DCOM- 20090430
LR  - 20141120
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 23
IP  - 2
DP  - 2007 Feb
TI  - [Specific cytotoxicity of CTL which was induced by DC pulsed with mannosylated 
      antigen].
PG  - 117-9
AB  - AIM: To investigate the role of mannosylated tumor antigen in the course of 
      anti-tumor response. METHODS: L2 domain of HER-2/neu (ErbB2) ectodomain was 
      expressed in E.coli, purified and mannosylated. Dendritic cells(DCs) were induced 
      from human peripheral blood mononuclear cell by GM-CSF and IL-4. The maturation 
      and functional capacity of DCs pulsed with mannosylated L2 (mL2) protein was 
      investigated. RESULTS: L2 protein could induce DC maturation, which was 
      accompanied by elevated expression of MHC and costimulatory molecules. The effect 
      of mL2 protein on DC maturation was more remarkable than that of non-mannosylated 
      L2 protein. Furthermore, DCs pulsed with mL2 could stimulate high tumor cell 
      lysis by CTL. CONCLUSION: Our experiment provide the foundation for the study of 
      new tumor vaccine by mannosylation.
FAU - Zhong, Guo-cheng
AU  - Zhong GC
AD  - Department of Oncology, 452 Hospital, Chengdu 610021, China. zgc005@yahoo.com.cn
FAU - Xu, Ming
AU  - Xu M
FAU - Shi, Ming
AU  - Shi M
FAU - Hu, Mei-ru
AU  - Hu MR
FAU - Min, Min
AU  - Min M
FAU - Chen, Jian
AU  - Chen J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Cell Line, Tumor
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interleukin-4/pharmacology
MH  - Leukocytes, Mononuclear/cytology
MH  - Receptor, ErbB-2/genetics/*immunology/*metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - T-Lymphocytes, Cytotoxic/*drug effects
EDAT- 2007/02/09 09:00
MHDA- 2009/05/01 09:00
CRDT- 2007/02/09 09:00
PHST- 2007/02/09 09:00 [pubmed]
PHST- 2009/05/01 09:00 [medline]
PHST- 2007/02/09 09:00 [entrez]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Feb;23(2):117-9.