PMID- 17287331
OWN - NLM
STAT- MEDLINE
DCOM- 20071011
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 45
IP  - 4
DP  - 2007 Apr
TI  - Retrospective species identification of microsporidian spores in diarrheic fecal 
      samples from human immunodeficiency virus/AIDS patients by multiplexed 
      fluorescence in situ hybridization.
PG  - 1255-60
AB  - In order to assess the applicability of multiplexed fluorescence in situ 
      hybridization (FISH) assay for the clinical setting, we conducted retrospective 
      analysis of 110 formalin-stored diarrheic stool samples from human 
      immunodeficiency virus (HIV)/AIDS patients with intestinal microsporidiosis 
      collected between 1992 and 2003. The multiplexed FISH assay identified 
      microsporidian spores in 94 of 110 (85.5%) samples: 49 (52.1%) were positive for 
      Enterocytozoon bieneusi, 43 (45.8%) were positive for Encephalitozoon 
      intestinalis, 2 (2.1%) were positive for Encephalitozoon hellem, and 9 samples 
      (9.6%) contained both E. bieneusi and E. intestinalis spores. Quantitative spore 
      counts per ml of stool yielded concentration values from 3.5 x 10(3) to 4.4 x 
      10(5) for E. bieneusi (mean, 8.8 x 10(4)/ml), 2.3 x 10(2) to 7.8 x 10(4) (mean, 
      1.5 x 10(4)/ml) for E. intestinalis, and 1.8 x 10(2) to 3.6 x 10(2) for E. hellem 
      (mean, 2.7 x 10(2)/ml). Identification of microsporidian spores by multiplex FISH 
      assay was more sensitive than both Chromotrope-2R and CalcoFluor White M2R 
      stains; 85.5% versus 72.7 and 70.9%, respectively. The study demonstrated that 
      microsporidian coinfection in HIV/AIDS patients with intestinal microsporidiosis 
      is not uncommon and that formalin-stored fecal samples older than 10 years may 
      not be suitable for retrospective analysis by techniques targeting rRNA. 
      Multiplexed FISH assay is a reliable, quantitative fluorescence microscopy method 
      for the simultaneous identification of E. bieneusi, E. intestinalis, and E. 
      hellem, as well as Encephalitozoon cuniculi, spores in fecal samples and is a 
      useful tool for assessing spore shedding intensity in intestinal 
      microsporidiosis. The method can be used for epidemiological investigations and 
      applied in clinical settings.
FAU - Graczyk, Thaddeus K
AU  - Graczyk TK
AD  - Department of Environmental Health Sciences, Division of Environmental Health 
      Engineering, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe 
      Street, Baltimore, MD 21205, USA. tgraczyk@jhsph.edu
FAU - Johansson, Michael A
AU  - Johansson MA
FAU - Tamang, Leena
AU  - Tamang L
FAU - Visvesvara, Govinda S
AU  - Visvesvara GS
FAU - Moura, Laci S
AU  - Moura LS
FAU - DaSilva, Alexandre J
AU  - DaSilva AJ
FAU - Girouard, Autumn S
AU  - Girouard AS
FAU - Matos, Olga
AU  - Matos O
LA  - eng
GR  - P30 ES003819/ES/NIEHS NIH HHS/United States
GR  - P30 ES03819/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070207
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
SB  - IM
MH  - AIDS-Related Opportunistic Infections/*diagnosis/microbiology
MH  - Colony Count, Microbial
MH  - Diarrhea/*microbiology
MH  - Encephalitozoon/classification/*isolation & purification
MH  - Encephalitozoonosis/diagnosis/microbiology
MH  - Enterocytozoon/classification/*isolation & purification
MH  - Feces/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Fluorescence
MH  - Microsporidiosis/*diagnosis/microbiology
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - Spores, Fungal/isolation & purification
PMC - PMC1865804
EDAT- 2007/02/09 09:00
MHDA- 2007/10/12 09:00
PMCR- 2007/08/01
CRDT- 2007/02/09 09:00
PHST- 2007/02/09 09:00 [pubmed]
PHST- 2007/10/12 09:00 [medline]
PHST- 2007/02/09 09:00 [entrez]
PHST- 2007/08/01 00:00 [pmc-release]
AID - JCM.01975-06 [pii]
AID - 1975-06 [pii]
AID - 10.1128/JCM.01975-06 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2007 Apr;45(4):1255-60. doi: 10.1128/JCM.01975-06. Epub 2007 
      Feb 7.