PMID- 17287465
OWN - NLM
STAT- MEDLINE
DCOM- 20070719
LR  - 20071115
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 56
IP  - 4
DP  - 2007 Apr
TI  - Characterization of donor dendritic cells and enhancement of dendritic cell 
      efflux with CC-chemokine ligand 21: a novel strategy to prolong islet allograft 
      survival.
PG  - 912-20
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells, yet little 
      data are available on the differential characteristics of donor and recipient DCs 
      (dDCs and rDCs, respectively) during the process of islet allograft rejection. 
      DTR-GFP-DC mice provide a novel tool to monitor DC trafficking and 
      characteristics during allograft rejection. We show rapid migration of dDCs to 
      recipient lymphoid tissues as early as 3 h post-islet allotransplantation. 
      Compared with rDCs, dDCs express different patterns of chemokine receptors, 
      display differential proliferative capacity, and exhibit a higher level of 
      maturity; these findings could be attributed to the effects of injury that dDCs 
      undergo during islet cell preparation and engraftment. Intriguingly, we detected 
      dDCs in the spleen of recipients long after rejection of islet allografts. Given 
      that dDCs express high levels of CCR7, islets were cultured before transplant 
      with the ligand for CCR7 (CCL21). This novel method, which enabled us to enhance 
      the efflux of dDCs from islet preparations, resulted in a prolongation of islet 
      allograft survival in immunocompetent recipients. This study introduces dDCs and 
      rDCs as two distinct types of DCs and provides novel data with clinical 
      implications to use chemokine-based DC-depleting strategies to prolong islet 
      allograft survival.
FAU - Fiorina, Paolo
AU  - Fiorina P
AD  - Transplantation Research Center (TRC), Children's Hospital and Brigham and 
      Women's Hospital, Harvard Medical School, 221 Longwood Ave., Boston, MA 02115, 
      USA.
FAU - Jurewicz, Mollie
AU  - Jurewicz M
FAU - Tanaka, Katsunori
AU  - Tanaka K
FAU - Behazin, Negin
AU  - Behazin N
FAU - Augello, Andrea
AU  - Augello A
FAU - Vergani, Andrea
AU  - Vergani A
FAU - von Andrian, Ulrich H
AU  - von Andrian UH
FAU - Smith, Neal R
AU  - Smith NR
FAU - Sayegh, Mohamed H
AU  - Sayegh MH
FAU - Abdi, Reza
AU  - Abdi R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070207
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (Ccl21c protein, mouse)
RN  - 0 (Chemokine CCL21)
RN  - 0 (Chemokines, CC)
SB  - IM
EIN - Diabetes. 2007 Jun;56(6):1747. Von Adrian, Uli [corrected to von Andrian, Ulrich 
      H]
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cell Division
MH  - Chemokine CCL21
MH  - Chemokines, CC/*physiology
MH  - Dendritic Cells/*cytology/drug effects/*transplantation
MH  - Diabetes Mellitus, Experimental/*surgery
MH  - Genes, Reporter
MH  - Islets of Langerhans Transplantation/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Transplantation, Homologous
EDAT- 2007/02/09 09:00
MHDA- 2007/07/20 09:00
CRDT- 2007/02/09 09:00
PHST- 2007/02/09 09:00 [pubmed]
PHST- 2007/07/20 09:00 [medline]
PHST- 2007/02/09 09:00 [entrez]
AID - db06-1445 [pii]
AID - 10.2337/db06-1445 [doi]
PST - ppublish
SO  - Diabetes. 2007 Apr;56(4):912-20. doi: 10.2337/db06-1445. Epub 2007 Feb 7.