PMID- 17288152
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 24
IP  - 6
DP  - 2006 Nov
TI  - [Hollow fiber liquid phase microextraction of tramadol from water and biological 
      samples].
PG  - 641-4
AB  - A novel technique of liquid phase microextraction supported by hollow fiber 
      membrane (LPME-HFM) was applied to extract tramadol in tap water, urine and human 
      plasma. At room temperature (20 degrees C), polyvinylidene fluoride hollow fiber 
      membrane (PVDF-HFM) was employed. In the procedure of extraction, 4 microL of 
      organic solvent (toluene) was used in individual extraction. Pethidine was used 
      as the internal standard (IS) in the sample analysis by gas chromatography with 
      flame ionization detector (GC-FID). The optimized conditions for tramadol 
      extraction was as follows: toluene as organic solvent, 4 mL sample solution added 
      with 0.1 mL of 1 mol/L NaOH solution, extraction time of 15 mm, and stirring 
      speed of 300 r/min. The sample solutions, containing a series of concentrations 
      of tramadol, 0.05, 0.10, 0.50, 1.0, 5.0, 10.0 mg/L, were analyzed. The good 
      linearities were obtained for water, human urine and human plasma samples with 
      the correlation coefficients of 0.999 7, 0.994 8 and 0.998 5, respectively. The 
      detection limits can reach 0.01 mg/L for water and human urine and 0.05 mg/L for 
      human plasma. The relative standard deviations (RSDs) were 10.6% and 5.15% for 
      water, 18. 1% and 4. 03% for urine solution at the spiked levels of 0.1 and 1.0 
      mg/L. The RSDs were 16.6% and 4.15% for plasma solution at the spiked levels of 
      0.5 and 1.0 mg/L. In comparison with conventional method, this method is simple, 
      sensitive, fast, inexpensive, and environmentally friendly. It can be applied to 
      pharmaceutical and forensic analysis.
FAU - Wang, Xiaofei
AU  - Wang X
AD  - (Pharmaceutical College, China Medical University, Shenyang 110001, China.
FAU - Liu, Junting
AU  - Liu J
FAU - Wang, Yan
AU  - Wang Y
FAU - Zhao, Xiuli
AU  - Zhao X
FAU - Gao, Lina
AU  - Gao L
FAU - Xu, Donghui
AU  - Xu D
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Polyvinyls)
RN  - 24937-79-9 (polyvinylidene fluoride)
RN  - 39J1LGJ30J (Tramadol)
SB  - IM
MH  - Analgesics, Opioid/*analysis/blood/urine
MH  - Flame Ionization
MH  - Fresh Water/*analysis
MH  - Humans
MH  - Membranes, Artificial
MH  - Polyvinyls
MH  - Tramadol/*isolation & purification
EDAT- 2007/02/10 09:00
MHDA- 2010/06/04 06:00
CRDT- 2007/02/10 09:00
PHST- 2007/02/10 09:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
PHST- 2007/02/10 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2006 Nov;24(6):641-4.