PMID- 17288405
OWN - NLM
STAT- MEDLINE
DCOM- 20070420
LR  - 20121115
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 79
IP  - 6
DP  - 2007 Mar 15
TI  - Magnetophoretic immunoassay of allergen-specific IgE in an enhanced magnetic 
      field gradient.
PG  - 2214-20
AB  - We demonstrate a novel magnetophoretic immunoassay of allergen-specific 
      immunoglobulin E (IgE) based on the magnetophoretic deflection velocity of a 
      microbead that is proportional to the associated magnetic nanoparticles under 
      enhanced magnetic field gradient in a microchannel. In this detection scheme, two 
      types of house dust mites, Dermatophagoides farinae (D. farinae) and 
      Dermatophagoides pteronyssinus (D. pteronyssinus), were used as the model 
      allergens. Polystyrene microbeads were conjugated with each of the mite extracts 
      followed by incubation with serum samples. The resulting mixture was then reacted 
      with magnetic nanoparticle-conjugated anti-human IgE for detection of 
      allergen-specific IgE by using sandwich immuno-reactions. A ferromagnetic 
      microstructure combined with a permanent magnet was employed to increase the 
      magnetic field gradient ( approximately 10(4) T/m) in a microfluidic device. The 
      magnetophoretic velocities of microbeads were measured in a microchannel under 
      applied magnetic field, and the averaged velocity was well correlated with the 
      concentration of allergen-specific IgE in serum. From the analysis of pooled sera 
      obtained from 44 patients, the detection limits of the allergen-specific human 
      IgEs for D. farinae and D. pteronyssinus were determined to be 565 (0.045 IU/mL) 
      and 268 fM (0.021 IU/mL), respectively. These values are 1 order of magnitude 
      lower than those by a conventional CAP system. For evaluation of reproducibility 
      and accuracy, unknown sera were subjected to a blind test by using the developed 
      assay system, and they were compared with the CAP system. As a result, 
      coefficient of variance was less than 10%, and the developed method enabled a 
      fast assay with a tiny amount of serum ( approximately 10 microL).
FAU - Hahn, Young Ki
AU  - Hahn YK
AD  - Department of BioSystems, Korea Advanced Institute of Science and Technology, 
      Daejeon 305-701, Korea.
FAU - Jin, Zongwen
AU  - Jin Z
FAU - Kang, Joo H
AU  - Kang JH
FAU - Oh, Eunkeu
AU  - Oh E
FAU - Han, Min-Kyu
AU  - Han MK
FAU - Kim, Hak-Sung
AU  - Kim HS
FAU - Jang, Jung-Tak
AU  - Jang JT
FAU - Lee, Jae-Hyun
AU  - Lee JH
FAU - Cheon, Jinwoo
AU  - Cheon J
FAU - Kim, Seung Hyun
AU  - Kim SH
FAU - Park, Hae-Sim
AU  - Park HS
FAU - Park, Je-Kyun
AU  - Park JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070209
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Allergens)
RN  - 0 (Aminoglycosides)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9Z22C3QQCJ (nikkomycin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens/*immunology
MH  - Aminoglycosides/chemistry
MH  - Animals
MH  - Antibody Specificity
MH  - Child
MH  - Child, Preschool
MH  - Goats
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/*blood/*immunology
MH  - *Magnetics
MH  - Middle Aged
MH  - Mites/immunology
EDAT- 2007/02/10 09:00
MHDA- 2007/04/21 09:00
CRDT- 2007/02/10 09:00
PHST- 2007/02/10 09:00 [pubmed]
PHST- 2007/04/21 09:00 [medline]
PHST- 2007/02/10 09:00 [entrez]
AID - 10.1021/ac061522l [doi]
PST - ppublish
SO  - Anal Chem. 2007 Mar 15;79(6):2214-20. doi: 10.1021/ac061522l. Epub 2007 Feb 9.