PMID- 17289045
OWN - NLM
STAT- MEDLINE
DCOM- 20070815
LR  - 20191210
IS  - 0016-6480 (Print)
IS  - 0016-6480 (Linking)
VI  - 152
IP  - 2-3
DP  - 2007 Jun-Jul
TI  - Hormone receptors in gills of smolting Atlantic salmon, Salmo salar: expression 
      of growth hormone, prolactin, mineralocorticoid and glucocorticoid receptors and 
      11beta-hydroxysteroid dehydrogenase type 2.
PG  - 295-303
AB  - This is the first study to report concurrent dynamics in mRNA expression of 
      growth hormone receptor (GHR), prolactin receptor (PRLR), gluco- and 
      mineralocorticoid receptor (GR and MR) and the 11beta-hydroxysteroid 
      dehydrogenase type-2 enzyme (11beta-HSD2) in Atlantic salmon (Salmo salar) gill 
      during smoltification. Transcript levels were analysed by quantitative PCR in 
      fresh water (FW) fish and after a 24-h salt water (SW) challenge. GHR transcript 
      levels increased concurrent with gill Na(+),K(+)-ATPase activity in FW fish 
      consistent with the SW-adaptive role of GH. SW-transfer induced an increased GHR 
      expression levels in the early stages of smoltification but a decrease in 
      expression at the peak of smoltification. PRLR transcript levels decreased 
      steadily during smoltification in agreement with the recognized 
      hyper-osmoregulatory role of PRL. Surprisingly, PRLR levels increased after SW 
      transfer during the course of smoltification. GR mRNA levels were low early on 
      during smoltification but increased at the peak of smoltification and remained 
      high during de-smoltification, indicative of increased cortisol signalling at 
      this point. Coherently, SW transfer increased GR levels to smolt levels prior to 
      the smoltification peak. 11beta-HSD2 levels increased at the smoltification peak 
      and MR levels increased during de-smoltification, suggesting a need for 
      protection of MR from cortisol signalling during smoltification. This is 
      supported by the fact that SW-transfer results in a profound up-regulation of 
      11beta-HSD2, whereas SW transfer down-regulates MR levels. The study concludes 
      that GR and MR may have distinctive roles in developing hypo- and 
      hyper-osmoregulatory mechanisms during smoltification and de-smoltification, 
      respectively.
FAU - Kiilerich, Pia
AU  - Kiilerich P
AD  - Institute of Biology, University of Southern Denmark, Campusvej 55, DK-5230 
      Odense M, Denmark.
FAU - Kristiansen, Karsten
AU  - Kristiansen K
FAU - Madsen, Steffen S
AU  - Madsen SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061230
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Mineralocorticoid)
RN  - 0 (Receptors, Prolactin)
RN  - 0 (Receptors, Somatotropin)
RN  - 0 (Receptors, Steroid)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenase Type 2)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenase Type 2/*genetics
MH  - Acclimatization/physiology
MH  - Animals
MH  - Gills/*physiology
MH  - RNA, Messenger/metabolism
MH  - Receptors, Glucocorticoid/genetics
MH  - Receptors, Mineralocorticoid/genetics
MH  - Receptors, Prolactin/genetics
MH  - Receptors, Somatotropin/genetics
MH  - Receptors, Steroid/*genetics
MH  - Salmo salar/*physiology
MH  - Sodium-Potassium-Exchanging ATPase/metabolism
MH  - Water-Electrolyte Balance/*physiology
EDAT- 2007/02/10 09:00
MHDA- 2007/08/19 09:00
CRDT- 2007/02/10 09:00
PHST- 2006/09/15 00:00 [received]
PHST- 2006/11/29 00:00 [revised]
PHST- 2006/12/26 00:00 [accepted]
PHST- 2007/02/10 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2007/02/10 09:00 [entrez]
AID - S0016-6480(06)00403-5 [pii]
AID - 10.1016/j.ygcen.2006.12.018 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2007 Jun-Jul;152(2-3):295-303. doi: 
      10.1016/j.ygcen.2006.12.018. Epub 2006 Dec 30.