PMID- 17289085 OWN - NLM STAT- MEDLINE DCOM- 20070425 LR - 20220310 IS - 0024-3205 (Print) IS - 0024-3205 (Linking) VI - 80 IP - 15 DP - 2007 Mar 20 TI - Antidepressant like effects of piperine in chronic mild stress treated mice and its possible mechanisms. PG - 1373-81 AB - In this study, we investigated the antidepressant-like effect of piperine in mice exposed to chronic mild stress (CMS) procedure. Repeated administration of piperine for 14 days at the doses of 2.5, 5 and 10 mg/kg reversed the CMS-induced changes in sucrose consumption, plasma corticosterone level and open field activity. Furthermore, the decreased proliferation of hippocampal progenitor cells was ameliorated and the level of brain-derived neurotrophic factor (BDNF) in hippocampus of CMS stressed mice was up-regulated by piperine treatment in the same time course. In addition, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactic dehydrogenase (LDH) assays showed that piperine (6.25-25 microM) or fluoxetine (FLU, 1 microM) dose-dependently protected primary cultured hippocampal neurons from the lesion induced by 10 microM corticosterone (CORT). Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the messenger ribonucleic acid (mRNA) level of BDNF in cultured neurons. Treatment with piperine (6.25-25 microM) for 72 h reversed the CORT-induced reduction of BDNF mRNA expression in cultured hippocampal neurons. In summary, up-regulation of the progenitor cell proliferation of hippocampus and cytoprotective activity might be mechanisms involved in the antidepressant-like effect of piperine, which may be closely related to the elevation of hippocampal BDNF level. FAU - Li, Song AU - Li S AD - Laboratory for Brain and Mind, Institute of Neuroinformatics, Dalian University of Technology, Dalian, 116023,China. lisong_spu2005@yahoo.com FAU - Wang, Che AU - Wang C FAU - Wang, Minwei AU - Wang M FAU - Li, Wei AU - Li W FAU - Matsumoto, Kinzo AU - Matsumoto K FAU - Tang, Yiyuan AU - Tang Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070112 PL - Netherlands TA - Life Sci JT - Life sciences JID - 0375521 RN - 0 (Alkaloids) RN - 0 (Antidepressive Agents) RN - 0 (Benzodioxoles) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Piperidines) RN - 0 (Polyunsaturated Alkamides) RN - 0 (Tetrazolium Salts) RN - 0 (Thiazoles) RN - EC 1.1.1.27 (L-Lactate Dehydrogenase) RN - EUY85H477I (thiazolyl blue) RN - U71XL721QK (piperine) RN - W980KJ009P (Corticosterone) SB - IM MH - Alkaloids/*pharmacology MH - Animals MH - *Antidepressive Agents MH - Benzodioxoles/*pharmacology MH - Brain-Derived Neurotrophic Factor/blood/metabolism MH - Cell Proliferation/drug effects MH - Cell Survival/drug effects MH - Cells, Cultured MH - Chronic Disease MH - Corticosterone/blood/pharmacology MH - Depression/*drug therapy/etiology/*psychology MH - Hippocampus/pathology MH - Immunohistochemistry MH - L-Lactate Dehydrogenase/metabolism MH - Male MH - Mice MH - Neurons/drug effects MH - Piperidines/*pharmacology MH - Polyunsaturated Alkamides/*pharmacology MH - Rats MH - Rats, Sprague-Dawley MH - Reverse Transcriptase Polymerase Chain Reaction MH - Stem Cells/drug effects MH - Stress, Psychological/*complications/pathology/*psychology MH - Tetrazolium Salts MH - Thiazoles MH - Weight Gain EDAT- 2007/02/10 09:00 MHDA- 2007/04/26 09:00 CRDT- 2007/02/10 09:00 PHST- 2006/09/27 00:00 [received] PHST- 2006/12/13 00:00 [revised] PHST- 2006/12/15 00:00 [accepted] PHST- 2007/02/10 09:00 [pubmed] PHST- 2007/04/26 09:00 [medline] PHST- 2007/02/10 09:00 [entrez] AID - S0024-3205(07)00041-0 [pii] AID - 10.1016/j.lfs.2006.12.027 [doi] PST - ppublish SO - Life Sci. 2007 Mar 20;80(15):1373-81. doi: 10.1016/j.lfs.2006.12.027. Epub 2007 Jan 12.