PMID- 17289434
OWN - NLM
STAT- MEDLINE
DCOM- 20070605
LR  - 20090422
IS  - 1472-9792 (Print)
IS  - 1472-9792 (Linking)
VI  - 87
IP  - 2
DP  - 2007 Mar
TI  - Mycobacterium bovis BCG vaccination modulates TNF-alpha production after 
      pulmonary challenge with virulent Mycobacterium tuberculosis in guinea pigs.
PG  - 155-65
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays critical and opposing roles in the 
      pathogenesis of tuberculosis (TB). We examined the effects of Mycobacterium bovis 
      BCG vaccination on TNF-alpha production in three distinct guinea pig leukocyte 
      populations before and after pulmonary infection with M. tuberculosis H37Rv. 
      Following BCG vaccination alone, and following challenge, bronchoalveolar lavage 
      cells (BALC), resident peritoneal cells (PC), and splenocytes (SPC) were 
      stimulated with purified protein derivative (PPD). Before virulent challenge, BCG 
      vaccination clearly enhanced the ability of BALC, PC and SPC to produce TNF-alpha 
      in response to PPD stimulation ex vivo. Following challenge, the TNF-alpha 
      production of all three leukocyte populations from BCG-vaccinated animals 
      remained relatively constant at pre-challenged levels. In sharp contrast, 5 weeks 
      post-challenge, all three leukocyte populations from unvaccinated animals 
      produced very high amounts of TNF-alpha in response to PPD. Three weeks 
      post-challenge, SPC from one of the unvaccinated animals produced higher levels 
      of TNF-alpha but the others produced lower levels of TNF-alpha than 
      BCG-vaccinated animals. As expected, BCG vaccination reduced the levels of 
      virulent mycobacteria in both the lungs and spleens. Thus, BCG vaccination allows 
      guinea pigs to modulate TNF-alpha levels in conjunction with a reduction in 
      bacillary loads in their tissues.
FAU - Yamamoto, Toshiko
AU  - Yamamoto T
AD  - Department of Microbial and Molecular Pathogenesis, The Texas A&M University 
      System Health Science Center, College Station, TX 77843-1114, USA.
FAU - Lasco, Todd M
AU  - Lasco TM
FAU - Uchida, Kazuyuki
AU  - Uchida K
FAU - Goto, Yoshitaka
AU  - Goto Y
FAU - Jeevan, Amminikutty
AU  - Jeevan A
FAU - McFarland, Christine
AU  - McFarland C
FAU - Ly, Lan
AU  - Ly L
FAU - Yamamoto, Saburo
AU  - Yamamoto S
FAU - McMurray, David N
AU  - McMurray DN
LA  - eng
GR  - R01 AI 15495/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070207
PL  - Scotland
TA  - Tuberculosis (Edinb)
JT  - Tuberculosis (Edinburgh, Scotland)
JID - 100971555
RN  - 0 (Aerosols)
RN  - 0 (BCG Vaccine)
RN  - 0 (Tuberculin)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aerosols
MH  - Animals
MH  - BCG Vaccine/*immunology
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - Guinea Pigs
MH  - Leukocytes/immunology
MH  - Lung/*immunology
MH  - Mycobacterium bovis/*immunology
MH  - Mycobacterium tuberculosis/*immunology
MH  - Peritoneal Cavity/pathology
MH  - Spleen/immunology/pathology
MH  - Tuberculin/immunology
MH  - Tuberculosis, Pulmonary/*immunology
MH  - Tumor Necrosis Factor-alpha/*immunology
MH  - Virulence
EDAT- 2007/02/10 09:00
MHDA- 2007/06/06 09:00
CRDT- 2007/02/10 09:00
PHST- 2006/02/24 00:00 [received]
PHST- 2006/06/05 00:00 [revised]
PHST- 2006/07/13 00:00 [accepted]
PHST- 2007/02/10 09:00 [pubmed]
PHST- 2007/06/06 09:00 [medline]
PHST- 2007/02/10 09:00 [entrez]
AID - S1472-9792(06)00087-4 [pii]
AID - 10.1016/j.tube.2006.07.002 [doi]
PST - ppublish
SO  - Tuberculosis (Edinb). 2007 Mar;87(2):155-65. doi: 10.1016/j.tube.2006.07.002. 
      Epub 2007 Feb 7.