PMID- 17289438
OWN - NLM
STAT- MEDLINE
DCOM- 20070604
LR  - 20161124
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 145
IP  - 2
DP  - 2007 Mar
TI  - Cytochrome P450 1A expression and organochlorine contaminants in harbour seals 
      (Phoca vitulina): evaluating a biopsy approach.
PG  - 256-64
AB  - We previously reported in vivo induction of cytochrome P450 1A (CYP1A) by 
      beta-naphthoflavone in skin and liver biopsies of captive harbour seals (Phoca 
      vitulina richardsi). The present study evaluated CYP1A expression (immunoblot 
      analysis and ethoxyresorufin O-deethylase activity-EROD) in harbour seals using 
      two study designs: i) skin and liver biopsies from 20 harbour seal pups captured 
      from coastal British Columbia (BC, Canada) and temporarily housed in captivity; 
      and ii) skin biopsies from 42 free-ranging harbour seals captured and sampled 
      on-site in multiple locations in BC and Washington State (USA). Toxic Equivalency 
      Quotients (TEQs) were calculated for polychlorinated biphenyl, polychlorinated 
      dibenzo-p-dioxin, and polychlorinated dibenzofuran residues measured in blubber 
      from a subset of study animals (n=30). CYP1A data from the seal pups held 
      temporarily in captivity show that CYP1A protein levels were greater in liver 
      than skin and that CYP1A protein and EROD activity were correlated in skin and 
      liver. However, analysis of free-ranging seals from different sites revealed that 
      blubber organochlorine TEQ values did not correlate with skin CYP1A levels. CYP1A 
      protein levels and EROD activities in skin of seal pups from the BC locations and 
      from Puget Sound were relatively low, possibly reflecting contaminant levels that 
      were not high enough to elicit a response, a small sample size, or methodological 
      limitations. Our results show that CYP1A measurements in skin show promise as a 
      biomarker of contaminant exposure, but that refinements to techniques and a 
      larger sample size are needed.
FAU - Assuncao, Marta G L
AU  - Assuncao MG
AD  - Marine Environmental Quality Section, Institute of Ocean Sciences, Fisheries and 
      Oceans Canada, P.O. Box 6000, 9860 West Saanich Road, Sidney, BC, Canada.
FAU - Miller, Kelsey A
AU  - Miller KA
FAU - Dangerfield, Neil J
AU  - Dangerfield NJ
FAU - Bandiera, Stelvio M
AU  - Bandiera SM
FAU - Ross, Peter S
AU  - Ross PS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070112
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Benzofurans)
RN  - 0 (Dibenzofurans, Polychlorinated)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Water Pollutants, Chemical)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Adipose Tissue/metabolism
MH  - Animals
MH  - Benzofurans/metabolism
MH  - Cytochrome P-450 CYP1A1/*metabolism
MH  - Dibenzofurans, Polychlorinated
MH  - Environmental Monitoring/methods
MH  - Female
MH  - Liver/*enzymology
MH  - Male
MH  - Phoca/*metabolism
MH  - Polychlorinated Biphenyls/metabolism
MH  - Polychlorinated Dibenzodioxins/analogs & derivatives/metabolism
MH  - Skin/*enzymology
MH  - Water Pollutants, Chemical/metabolism
EDAT- 2007/02/10 09:00
MHDA- 2007/06/05 09:00
CRDT- 2007/02/10 09:00
PHST- 2006/09/29 00:00 [received]
PHST- 2006/12/22 00:00 [revised]
PHST- 2006/12/28 00:00 [accepted]
PHST- 2007/02/10 09:00 [pubmed]
PHST- 2007/06/05 09:00 [medline]
PHST- 2007/02/10 09:00 [entrez]
AID - S1532-0456(07)00008-7 [pii]
AID - 10.1016/j.cbpc.2006.12.015 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2007 Mar;145(2):256-64. doi: 
      10.1016/j.cbpc.2006.12.015. Epub 2007 Jan 12.