PMID- 17289932 OWN - NLM STAT- MEDLINE DCOM- 20070516 LR - 20161124 IS - 1066-5099 (Print) IS - 1066-5099 (Linking) VI - 25 IP - 5 DP - 2007 May TI - Cardiac stem cells in brown adipose tissue express CD133 and induce bone marrow nonhematopoietic cells to differentiate into cardiomyocytes. PG - 1326-33 AB - Recently, there has been noteworthy progress in the field of cardiac regeneration therapy. We previously reported that brown adipose tissue (BAT) contained cardiac progenitor cells that were relevant to the regeneration of damaged myocardium. In this study, we found that CD133-positive, but not c-Kit- or Sca-1-positive, cells in BAT differentiated into cardiomyocytes (CMs) with a high frequency. Moreover, we found that CD133(+) brown adipose tissue-derived cells (BATDCs) effectively induced bone marrow cells (BMCs) into CMs. BMCs are considered to have the greatest potential as a source of CMs, and two sorts of stem cell populations, the MSCs and hematopoietic stem cells (HSCs), have been reported to differentiate into CMs; however, it has not been determined which population is a better source of CMs. Here we show that CD133-positive BATDCs induce BMCs into CMs, not through cell fusion but through bivalent cation-mediated cell-to-cell contact when cocultured. Moreover, BMCs induced by BATDCs are able to act as CM repletion in an in vivo infarction model. Finally, we found that CD45(-)CD31(-) CD105(+) nonhematopoietic cells, when cocultured with BATDCs, generated more than 20 times the number of CMs compared with lin(-)c-Kit(+) HSCs. Taken together, these data suggest that CD133-positive BATDCs are a useful tool as CM inducers, as well as a source of CMs, and that the nonhematopoietic fraction in bone marrow is also a major source of CMs. Disclosure of potential conflicts of interest is found at the end of this article. FAU - Yamada, Yoshihiro AU - Yamada Y AD - Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, Suita-shi, Osaka, Japan. FAU - Yokoyama, Shin-ichiro AU - Yokoyama S FAU - Wang, Xiang-Di AU - Wang XD FAU - Fukuda, Noboru AU - Fukuda N FAU - Takakura, Nobuyuki AU - Takakura N LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070208 PL - England TA - Stem Cells JT - Stem cells (Dayton, Ohio) JID - 9304532 RN - 0 (AC133 Antigen) RN - 0 (Antigens, CD) RN - 0 (Cations, Divalent) RN - 0 (Glycoproteins) RN - 0 (Peptides) RN - 0 (Prom1 protein, mouse) RN - 0 (Prom1 protein, rat) SB - IM MH - AC133 Antigen MH - Adipose Tissue, Brown/*cytology/drug effects MH - Animals MH - Antigens, CD/*metabolism MH - Bone Marrow Cells/*cytology/drug effects MH - Cations, Divalent/pharmacology MH - Cell Communication/drug effects MH - *Cell Differentiation/drug effects MH - Cell Fusion MH - Coculture Techniques MH - Glycoproteins/*metabolism MH - Leukocytes, Mononuclear/cytology MH - Mice MH - Mice, Inbred C57BL MH - Myocytes, Cardiac/*cytology/drug effects MH - Peptides/*metabolism MH - Phenotype MH - Rats MH - Rats, Sprague-Dawley MH - Regeneration/drug effects MH - Stem Cells/*cytology/drug effects EDAT- 2007/02/10 09:00 MHDA- 2007/05/17 09:00 CRDT- 2007/02/10 09:00 PHST- 2007/02/10 09:00 [pubmed] PHST- 2007/05/17 09:00 [medline] PHST- 2007/02/10 09:00 [entrez] AID - 2006-0588 [pii] AID - 10.1634/stemcells.2006-0588 [doi] PST - ppublish SO - Stem Cells. 2007 May;25(5):1326-33. doi: 10.1634/stemcells.2006-0588. Epub 2007 Feb 8.