PMID- 17290352
OWN - NLM
STAT- MEDLINE
DCOM- 20070322
LR  - 20170227
IS  - 1699-5848 (Electronic)
IS  - 0213-3911 (Linking)
VI  - 22
IP  - 4
DP  - 2007 Apr
TI  - Histopathological alterations, EROD activity, CYP1A protein and biliary 
      metabolites in gilthead seabream Sparus aurata exposed to benzo(a)pyrene.
PG  - 417-32
LID - 10.14670/HH-22.417 [doi]
AB  - This study compared for seabream, Sparus aurata exposed to benzo(a)pyrene-B(a)P-, 
      the response of molecular cytochrome P450 1A (CYP1A) and cellular histopathology 
      biomarkers. Male gilthead seabream, Sparus aurata specimens were exposed for 20 
      days via water to a series of high B(a)P concentrations. CYP1A was assessed by 
      measuring enzymatic activity (EROD) and CYP1A protein content, and cellular 
      responses were evaluated by routine histopathological methods. In addition, 
      biliary metabolites were measured in order to verify that B(a)P was absorbed and 
      metabolised. Histological lesions, both in liver and gills, increased in parallel 
      to B(a)P concentrations, with the majority of changes representing rather 
      non-specific alterations. Hepatic EROD and CYP1A proteins data showed a 
      concentration-dependent induction, while in the gills, EROD activity but not 
      CYP1A proteins showed a non-monotonous dose response, with a maximum induction 
      level at 200 microg B(a)P.L-1 and decreasing levels thereafter. The findings 
      provide evidence that short-term, high dose exposure of fish can result in 
      significant uptake and metabolism of the lipophilic B(a)P, and in pronounced 
      pathological damage of absorptive epithelia and internal organs.
FAU - Ortiz-Delgado, J B
AU  - Ortiz-Delgado JB
AD  - Institute of Marine Sciences of Andalucia, CSIC, Poligono Rio San Pedro, Puerto 
      Real, Cadiz, Spain.
FAU - Segner, H
AU  - Segner H
FAU - Arellano, J M
AU  - Arellano JM
FAU - Sarasquete, C
AU  - Sarasquete C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (Biomarkers)
RN  - 0 (Water Pollutants, Chemical)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Benzo(a)pyrene/*toxicity
MH  - Bile/*drug effects/enzymology
MH  - Biomarkers/metabolism
MH  - Cytochrome P-450 CYP1A1/*metabolism
MH  - Cytoplasm/drug effects/ultrastructure
MH  - Dose-Response Relationship, Drug
MH  - Gills/*drug effects/pathology/ultrastructure
MH  - Liver/*drug effects/pathology/ultrastructure
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - *Sea Bream
MH  - Water Pollutants, Chemical/*toxicity
EDAT- 2007/02/10 09:00
MHDA- 2007/03/23 09:00
CRDT- 2007/02/10 09:00
PHST- 2007/02/10 09:00 [pubmed]
PHST- 2007/03/23 09:00 [medline]
PHST- 2007/02/10 09:00 [entrez]
AID - 10.14670/HH-22.417 [doi]
PST - ppublish
SO  - Histol Histopathol. 2007 Apr;22(4):417-32. doi: 10.14670/HH-22.417.