PMID- 17293344
OWN - NLM
STAT- MEDLINE
DCOM- 20070709
LR  - 20181201
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 22
IP  - 5
DP  - 2007 May
TI  - Regulation of granulosa cell proliferation and EGF-like ligands during the 
      periovulatory interval in monkeys.
PG  - 1247-52
AB  - BACKGROUND: This study seeks to clarify cell cycle dynamics of granulosa cells 
      following hCG and elucidate the expression of epidermal growth factor (EGF)-like 
      ligands during luteinization. METHODS: Granulosa cells were obtained from rhesus 
      macaques undergoing controlled ovarian stimulation protocols before or after an 
      ovulatory hCG bolus. Cell cycle characteristics were determined by flow cytometry 
      and levels of EGF receptor (EGFR), amphiregulin (AREG), epiregulin (EREG) and 
      betacellulin (BTC) mRNAs were measured by real-time RT-PCR. RESULTS: The 
      proportion of cells in S-phase was 7.5% prior to hCG and did not decline until 24 
      h after hCG (3.1%). EGFR protein and BTC mRNA did not change following hCG, 
      whereas AREG and EREG mRNA increased starting at 3 and 12 h post-hCG, 
      respectively, and remained elevated thereafter. CONCLUSIONS: Cell cycle transit 
      of macaque granulosa cells does not change until 24 h after an ovulatory 
      stimulus, whereas the EGF-like ligands EREG and AREG are increased rapidly. This 
      suggests that luteinizing granulosa cells are refractory to mitogenic stimulation 
      by EGFR ligands.
FAU - Fru, K N
AU  - Fru KN
AD  - Department of Physiology, Medical College of Georgia, Augusta, GA, USA.
FAU - Cherian-Shaw, M
AU  - Cherian-Shaw M
FAU - Puttabyatappa, M
AU  - Puttabyatappa M
FAU - VandeVoort, C A
AU  - VandeVoort CA
FAU - Chaffin, C L
AU  - Chaffin CL
LA  - eng
GR  - HD043358/HD/NICHD NIH HHS/United States
GR  - HD047964/HD/NICHD NIH HHS/United States
GR  - RR00169/RR/NCRR NIH HHS/United States
GR  - RR13439/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070209
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Amphiregulin)
RN  - 0 (Betacellulin)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Epiregulin)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Amphiregulin
MH  - Animals
MH  - Betacellulin
MH  - Cell Cycle/drug effects
MH  - Cell Proliferation/*drug effects
MH  - Chorionic Gonadotropin/pharmacology
MH  - Epidermal Growth Factor/*metabolism
MH  - Epiregulin
MH  - ErbB Receptors/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression Regulation/drug effects
MH  - Glycoproteins/*metabolism
MH  - Granulosa Cells/drug effects/*physiology
MH  - Intercellular Signaling Peptides and Proteins/*metabolism
MH  - Luteal Phase/metabolism
MH  - Macaca mulatta
MH  - Ovulation/*metabolism
MH  - Ovulation Induction/methods
MH  - RNA, Messenger/metabolism
EDAT- 2007/02/13 09:00
MHDA- 2007/07/10 09:00
CRDT- 2007/02/13 09:00
PHST- 2007/02/13 09:00 [pubmed]
PHST- 2007/07/10 09:00 [medline]
PHST- 2007/02/13 09:00 [entrez]
AID - del519 [pii]
AID - 10.1093/humrep/del519 [doi]
PST - ppublish
SO  - Hum Reprod. 2007 May;22(5):1247-52. doi: 10.1093/humrep/del519. Epub 2007 Feb 9.