PMID- 17296163
OWN - NLM
STAT- MEDLINE
DCOM- 20071218
LR  - 20121115
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 52
IP  - 7
DP  - 2007 Jul
TI  - Plasminogen activation by fibroblasts from periodontal ligament and gingiva is 
      not directly affected by chemokines in vitro.
PG  - 663-8
AB  - OBJECTIVE: Chronic inflammation in periodontal disease is associated with 
      increased plasminogen activation and elevated levels of chemokines. It is unknown 
      whether chemokines can regulate the activation of plasminogen via modulation of 
      plasminogen activators (PA) and the corresponding plasminogen activator 
      inhibitors (PAI) in periodontal tissue. DESIGN: To establish a link between 
      chemokines and activation of plasminogen, human periodontal ligament fibroblasts 
      (PDL) and gingival fibroblasts (GF) were incubated with IL-8, monocyte 
      chemoattractant protein-1, macrophage inflammatory protein-1alpha, and platelet 
      factor-4, either alone or in the presence of the inflammatory mediators TGF-beta 
      and IL-1. The potential of the cell lysates to activate plasminogen was based on 
      kinetic studies with the substrate casein. Casein zymography was performed to 
      determine the molecular sizes of the PA. Total PAI-1 in the cell-conditioned 
      medium was quantified by immunoassay. RESULTS: We report that the chemokines did 
      not affect activation of plasminogen by PDL and GF. Even in the presence of 
      TGF-beta which suppressed, and IL-1 which stimulated plasminogen activation, the 
      chemokines had no direct effect. Inhibition of PA and plasmin, but not of matrix 
      metalloproteinases and cysteine proteinases prevented caseinolysis. The 
      plasminogen activation capacity of the cell lysates was represented by a single 
      band with features of uPA. The immunoassay showed that the release of PAI-1 in 
      PDL and GF remained unaffected by the chemokines, also when stimulated with 
      TGF-beta. CONCLUSIONS: These results suggest that plasminogen activation by PDL 
      and GF is not directly affected by the chemokines even in the presence of the 
      inflammatory mediators TGF-beta and IL-1.
FAU - Sarajlic, Jasna
AU  - Sarajlic J
AD  - Department of Oral Surgery, Medical University of Vienna, Vienna, Austria, 
      Wahringerstrasse 25a, A-1090 Vienna, Austria.
FAU - Agis, Hermann
AU  - Agis H
FAU - Kandler, Barbara
AU  - Kandler B
FAU - Watzek, Georg
AU  - Watzek G
FAU - Gruber, Reinhard
AU  - Gruber R
LA  - eng
PT  - Journal Article
DEP - 20070212
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Caseins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokines)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Plasminogen Inactivators)
RN  - 0 (Transforming Growth Factor beta)
RN  - 37270-94-3 (Platelet Factor 4)
RN  - 9001-91-6 (Plasminogen)
RN  - EC 3.4.21.- (Plasminogen Activators)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Caseins/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/pharmacology
MH  - Chemokine CCL3/pharmacology
MH  - Chemokines/*pharmacology
MH  - Culture Media, Conditioned
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - Fibroblasts/drug effects/*metabolism
MH  - Gingiva/cytology/drug effects/*metabolism
MH  - Humans
MH  - Inflammation Mediators/pharmacology
MH  - Interleukin-1/pharmacology
MH  - Interleukin-8/pharmacology
MH  - Matrix Metalloproteinase Inhibitors
MH  - Periodontal Ligament/cytology/drug effects/*metabolism
MH  - Plasminogen/drug effects/*metabolism
MH  - Plasminogen Activators/analysis/drug effects
MH  - Plasminogen Inactivators/metabolism
MH  - Platelet Factor 4/pharmacology
MH  - Transforming Growth Factor beta/pharmacology
MH  - Urokinase-Type Plasminogen Activator/analysis
EDAT- 2007/02/14 09:00
MHDA- 2007/12/19 09:00
CRDT- 2007/02/14 09:00
PHST- 2006/07/28 00:00 [received]
PHST- 2006/12/04 00:00 [revised]
PHST- 2006/12/18 00:00 [accepted]
PHST- 2007/02/14 09:00 [pubmed]
PHST- 2007/12/19 09:00 [medline]
PHST- 2007/02/14 09:00 [entrez]
AID - S0003-9969(07)00022-2 [pii]
AID - 10.1016/j.archoralbio.2006.12.020 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2007 Jul;52(7):663-8. doi: 10.1016/j.archoralbio.2006.12.020. 
      Epub 2007 Feb 12.