PMID- 17298412
OWN - NLM
STAT- MEDLINE
DCOM- 20070508
LR  - 20121115
IS  - 0143-2885 (Print)
IS  - 0143-2885 (Linking)
VI  - 40
IP  - 4
DP  - 2007 Apr
TI  - The effect of chemical inhibition of matrix metalloproteinases on the size of 
      experimentally induced apical periodontitis.
PG  - 282-9
AB  - AIM: To determine the effect of matrix metalloproteinase (MMP) inhibition on 
      periapical lesion formation in a rat model. METHODOLOGY: The pulp chambers of 
      mandibular fist molars of adult SD rats were exposed to be infected by oral 
      microbes. The experimental group was fed 20 mg kg(-1) MMP-inhibitor chemically 
      modified tetracycline-3 (CMT-3) daily in an oral gavage and the controls were fed 
      the vehicle. After 2 and 4 weeks, the mandibles (n = 10 in both groups at both 
      times) were radiographed, decalcified and subjected to histological analysis. 
      Extension of necrosis in first molar distal root canals was measured from the 
      histological sections, and periapical lesion sizes in the same roots were 
      determined from radiographs and histological sections. Mann-Whitney U-test was 
      used for the statistical analysis. RESULTS: There was a statistically significant 
      difference in the extension of necrosis in root canals between 2 and 4 weeks in 
      the control group (P < 0.05), but not with MMP inhibition. Radiographically, MMP 
      inhibition increased the periapical lesion size by 70% and 34% after 2 and 4 
      weeks respectively (P < 0.05 in after 2 weeks). In histological measurements, 
      lesion size increased with MMP inhibition by 26% and 8% after 2 and 4 weeks 
      respectively. CONCLUSIONS: MMP inhibition affects pulpal and periapical 
      inflammation, increasing the rate of spreading of necrosis in root canals and the 
      rate of periapical lesion formation.
FAU - Tjaderhane, L
AU  - Tjaderhane L
AD  - Institute of Dentistry, University of Helsinki, Helsinki, Finland. 
      leo.tjaderhane@helsinki.fi
FAU - Hotakainen, T
AU  - Hotakainen T
FAU - Kinnunen, S
AU  - Kinnunen S
FAU - Ahonen, M
AU  - Ahonen M
FAU - Salo, T
AU  - Salo T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070208
PL  - England
TA  - Int Endod J
JT  - International endodontic journal
JID - 8004996
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Tetracyclines)
RN  - 0 (tetracycline CMT-3)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
EIN - Int Endod J. 2007 Jun;40(6):491
MH  - Animals
MH  - Bacteroidaceae Infections/enzymology
MH  - Dental Pulp Necrosis/*enzymology
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases/*physiology
MH  - Periapical Periodontitis/*enzymology
MH  - Porphyromonas gingivalis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tetracyclines/pharmacology
EDAT- 2007/02/15 09:00
MHDA- 2007/05/09 09:00
CRDT- 2007/02/15 09:00
PHST- 2007/02/15 09:00 [pubmed]
PHST- 2007/05/09 09:00 [medline]
PHST- 2007/02/15 09:00 [entrez]
AID - IEJ1223 [pii]
AID - 10.1111/j.0143-2885.2007.01223.x [doi]
PST - ppublish
SO  - Int Endod J. 2007 Apr;40(4):282-9. doi: 10.1111/j.0143-2885.2007.01223.x. Epub 
      2007 Feb 8.