PMID- 17298742
OWN - NLM
STAT- MEDLINE
DCOM- 20090924
LR  - 20171116
VI  - 26
IP  - 2
DP  - 2007 Feb
TI  - [Effects of soluble secreted by acute myeloid leukemia cells on differentiation, 
      maturation, apoptosis, and functions of dendritic cells].
PG  - 142-7
AB  - BACKGROUND & OBJECTIVE: Dendritic cells (DCs) play an important role in the 
      immunosurveillance against cancer. It has been shown that the function of DCs is 
      impaired and their population decreases in cancer-bearing hosts. Recent 
      observations suggest that the inability of DCs could be a result of the 
      immunosuppression mediated by soluble factors secreted by tumor cells. This study 
      was to investigate the effects of soluble factors secreted by acute myeloid 
      leukemia (AML) cells on the differentiation, maturation, apoptosis, and functions 
      of DCs derived from normal mononuclear cells. METHODS: Mononuclear cells were 
      cultured with interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating 
      factor (GM-CSF), in the presence or absence of 24-hour culture supernatants from 
      fresh primary AML cells, to generate immature DCs. The maturation of DCs was 
      induced by cytokines IL-1beta, IL-6, tumor necrosis factor-alpha (TNF-alpha), and 
      prostaglandin-2 (PGE-2). The phenotypic and apoptotic alterations of DCs were 
      evaluated using flow cytometry. Precursor frequency (PF) was calculated to 
      monitor the allostimulatory effects of DCs on CD4+ and CD8+ T cells. RESULTS: AML 
      cell supernatant-treated DCs showed significantly lower expression of 
      co-stimulatory molecules CD80 and CD86, and reduced response to cytokines 
      IL-1beta, IL-6, TNF-alpha, and PGE-2. The apoptosis rate was significantly lower 
      in AML cell supernatant-treated DCs than in control DCs [(29.4+/-9.5)% vs. 
      (15.1+/-4.2)%, P<0.01]. The allostimulatory effects of AML cell 
      supernatant-treated DCs on CD4+ and CD8+ T cells were significantly lower than 
      those of normal mature DCs [PF: (1.8+/-0.5)% vs. (5.2+/-1.6)% for CD4+ T cells, 
      (2.1+/-0.6)% vs. (6.5+/-2.0)% for CD8+ T cells, P<0.01]. CONCLUSION: Soluble 
      factors derived from AML cells could inhibit development and functions of DCs.
FAU - Wang, Xing-Bing
AU  - Wang XB
AD  - Department of Hematology, Anhui Provincial Hospital, Anhui Medical University, 
      Hefei, Anhui, PR China.
FAU - Liu, Jun
AU  - Liu J
FAU - Wu, Jing-Sheng
AU  - Wu JS
FAU - Sun, Zi-Min
AU  - Sun ZM
FAU - Huang, Shi-Ang
AU  - Huang SA
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Ai Zheng
JT  - Ai zheng = Aizheng = Chinese journal of cancer
JID - 9424852
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Culture Media)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - *Apoptosis
MH  - B7-1 Antigen/metabolism
MH  - B7-2 Antigen/metabolism
MH  - CD4-Positive T-Lymphocytes/cytology
MH  - CD8-Positive T-Lymphocytes/cytology
MH  - Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Culture Media
MH  - *Dendritic Cells/cytology/immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interleukin-4/pharmacology
MH  - Leukemia, Myeloid, Acute/*immunology/pathology
EDAT- 2007/02/15 09:00
MHDA- 2009/09/25 06:00
CRDT- 2007/02/15 09:00
PHST- 2007/02/15 09:00 [pubmed]
PHST- 2009/09/25 06:00 [medline]
PHST- 2007/02/15 09:00 [entrez]
AID - 1000-467X200702142 [pii]
PST - ppublish
SO  - Ai Zheng. 2007 Feb;26(2):142-7.