PMID- 17299251
OWN - NLM
STAT- MEDLINE
DCOM- 20070516
LR  - 20190513
IS  - 0449-3060 (Print)
IS  - 0449-3060 (Linking)
VI  - 48
IP  - 2
DP  - 2007 Mar
TI  - In vivo gamma-rays induced initial DNA damage and the effect of famotidine in 
      mouse leukocytes as assayed by the alkaline comet assay.
PG  - 129-34
AB  - Ionizing radiation induces a variety of lesions in DNA, each of which can be used 
      as a bio-indicator for biological dosimetry or the study of the radioprotective 
      effects of substances. To assess gamma ray-induced DNA damage in vivo in mouse 
      leukocytes at various doses and the effect of famotidine, blood was collected 
      from Balb/c male mice after irradiation with 4 Gy gamma-rays at different time 
      intervals post-irradiation. To assess the response, mice were irradiated with 
      doses of gamma-rays at 1 to 4 Grays. Famotidine was injected intra-peritoneally 
      (i.p) at a dose of 5 mg/kg at various time intervals before irradiation. Four 
      slides were prepared from each sample and alkaline comet assay was performed 
      using standard protocols. Results obtained show that radiation significantly 
      increases DNA damage in leukocytes in a dose dependent manner (p < 0.01) when 
      using appropriate sampling time after irradiation, because increasing sampling 
      time after irradiation resulted in a time dependent disappearance of DNA damage. 
      Treatment with only 5 mg/kg famotidine before 4 Gy irradiation led to almost 50% 
      reduction in DNA damage when compared with those animals which received radiation 
      alone. The radioprotective capability of famotidine might be attributed to 
      radical scavenging properties and an anti-oxidation mechanism.
FAU - Mozdarani, Hossein
AU  - Mozdarani H
AD  - Dept. of Medical Genetics, School of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran. mozdarah@modares.ac.ir
FAU - Nasirian, Borzo
AU  - Nasirian B
FAU - Haeri, S Abolghasem
AU  - Haeri SA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070214
PL  - England
TA  - J Radiat Res
JT  - Journal of radiation research
JID - 0376611
RN  - 0 (Anti-Ulcer Agents)
RN  - 5QZO15J2Z8 (Famotidine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Anti-Ulcer Agents/pharmacology
MH  - Comet Assay/*methods
MH  - DNA/chemistry
MH  - *DNA Damage
MH  - DNA Repair/radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Famotidine/*pharmacology
MH  - *Gamma Rays
MH  - Leukocytes/*drug effects/*radiation effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Radiation, Ionizing
EDAT- 2007/02/15 09:00
MHDA- 2007/05/17 09:00
CRDT- 2007/02/15 09:00
PHST- 2007/02/15 09:00 [pubmed]
PHST- 2007/05/17 09:00 [medline]
PHST- 2007/02/15 09:00 [entrez]
AID - JST.JSTAGE/jrr/06055 [pii]
AID - 10.1269/jrr.06055 [doi]
PST - ppublish
SO  - J Radiat Res. 2007 Mar;48(2):129-34. doi: 10.1269/jrr.06055. Epub 2007 Feb 14.