PMID- 17300894
OWN - NLM
STAT- MEDLINE
DCOM- 20071107
LR  - 20161124
IS  - 1872-6283 (Electronic)
IS  - 0379-0738 (Linking)
VI  - 172
IP  - 2-3
DP  - 2007 Oct 25
TI  - Time-resolved hair analysis of MDMA enantiomers by GC/MS-NCI.
PG  - 150-5
AB  - The aim of the study was to determine the enantioselective disposition of 
      3,4-methylenedioxymethamphetamine (MDMA) and other amphetamine-type stimulants 
      (ATS) in segmented hair specimens of self-declared ecstasy abusers, who took part 
      in a double-blind placebo-controlled six-way crossover study during approximately 
      7 weeks, during which they received a 75 and a 100 mg dose of racemic MDMA twice. 
      Hair specimens were washed and cut into pieces of 2 cm length. After digestion 
      and solid phase extraction, the enantiomers were derivatized with a chiral agent 
      (2S,4R)-N-heptafluorobutyryl-4-heptafluorobutoyloxy-prolyl chloride, developed at 
      the authors laboratory and quantified by gas chromatography coupled to mass 
      spectrometry operating in the negative chemical ionization mode. Most of the hair 
      specimens that were tested positive for MDMA showed a predominance of the 
      (R)-enantiomer. The R/S ratios of MDMA varied between 1.02 and 2.75 and total 
      concentrations ranged from 0.1 to 20.1 ng/mg. The enantiomers of its metabolite 
      3,4-methylenedioxyamphetamine (MDA) were also quantified in most hair segments. 
      The R/S ratios of MDA varied between 0.60 and 1.60, while the concentrations of 
      the enantiomers ranged from 10 to 160 pg/mg hair. When segmental analysis was 
      performed on single hair specimens, no inversion of the R versus S ratios of MDMA 
      and MDA was observed. The predominance of (R)-MDMA in hair was in accordance with 
      those already published for other matrices. Furthermore, both enantiomers of 
      amphetamine (AM) were also detected in hair segments of four volunteers and the 
      R/S ratios ranged from 1.00 to 1.47.
FAU - Martins, Liliane Ferreira
AU  - Martins LF
AD  - National Laboratory of Health, Toxicology Division, University of Luxembourg, 
      Luxembourg, Luxembourg. liliane.martins@lns.etat.lu <liliane.martins@lns.etat.lu>
FAU - Yegles, Michel
AU  - Yegles M
FAU - Samyn, Nele
AU  - Samyn N
FAU - Ramaekers, Johannes G
AU  - Ramaekers JG
FAU - Wennig, Robert
AU  - Wennig R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20070214
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Biomarkers)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - CK833KGX7E (Amphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - ML1I4KK67B (3,4-methylenedioxyethamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/analogs & derivatives/analysis
MH  - Amphetamine/analysis
MH  - Biomarkers/metabolism
MH  - Cross-Over Studies
MH  - Dose-Response Relationship, Drug
MH  - Double-Blind Method
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Hair/*chemistry
MH  - Humans
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*analysis
MH  - Substance Abuse Detection/*methods
MH  - Time Factors
EDAT- 2007/02/16 09:00
MHDA- 2007/11/08 09:00
CRDT- 2007/02/16 09:00
PHST- 2006/09/08 00:00 [received]
PHST- 2007/01/08 00:00 [revised]
PHST- 2007/01/13 00:00 [accepted]
PHST- 2007/02/16 09:00 [pubmed]
PHST- 2007/11/08 09:00 [medline]
PHST- 2007/02/16 09:00 [entrez]
AID - S0379-0738(07)00020-5 [pii]
AID - 10.1016/j.forsciint.2007.01.008 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2007 Oct 25;172(2-3):150-5. doi: 
      10.1016/j.forsciint.2007.01.008. Epub 2007 Feb 14.