PMID- 17302747
OWN - NLM
STAT- MEDLINE
DCOM- 20070315
LR  - 20220311
IS  - 0933-7407 (Print)
IS  - 0933-7407 (Linking)
VI  - 50
IP  - 1
DP  - 2007 Jan
TI  - Diagnosis of paracoccidioidomycosis by a dot blot assay using a recombinant 
      Paracoccidioides brasiliensis p27 protein.
PG  - 41-7
AB  - A variety of immunological methods have proven useful for Paracoccidioidomycosis 
      (PCM) diagnosis; however, they are often time consuming and many lack sensitivity 
      and specificity, partially attributed to the use of crude antigens, which give 
      cross reactivity. Until now, attempts to clone and express Paracoccidioides 
      brasiliensis immunodominant antigens have presented difficulties of process and 
      problems of cost. In an attempt to obtain a more rapid, sensitive, and specific 
      test for PCM diagnosis, we subcloned the P. brasiliensis p27 gene and used the 
      recombinant protein as the antigen in dot blot assays to evaluate its usefulness 
      in paracoccidioidomicosis diagnosis. The development of an optimised procedure 
      for p27 recombinant protein purification and production led to an easier and less 
      expensive process than the one previously used in our laboratory and allowed the 
      availability of enough purified protein for its evaluation as the antigen in the 
      dot blot assays. In these assays, antibodies present in ten serum samples from 
      seven patients with PCM recognised the recombinant protein showing a sensitivity 
      of 100% with a specificity of 98%. These results confirm the value of the 27-kDa 
      recombinant antigen in the serodiagnosis of paracoccidioidomycosis and that the 
      dot blot format is an alternative to the immunoenzymatic assay procedure.
FAU - Correa, M M
AU  - Correa MM
AD  - Grupo de Microbiologia Molecular, Escuela de Bacteriologia y Laboratorio Clinico, 
      Universidad de Antioquia, Medellin, Colombia. mcorrea@quimbaya.udea.edu.co
FAU - Bedoya, A M
AU  - Bedoya AM
FAU - Guerrero, M P
AU  - Guerrero MP
FAU - Mendez, J
AU  - Mendez J
FAU - Restrepo, A
AU  - Restrepo A
FAU - McEwen, J G
AU  - McEwen JG
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Mycoses
JT  - Mycoses
JID - 8805008
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Antibodies, Fungal/*blood
MH  - Antigens, Fungal/genetics/*immunology
MH  - Blotting, Western
MH  - Fungal Proteins/genetics/*immunology
MH  - Humans
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*diagnosis/microbiology
MH  - Recombinant Proteins/genetics/*immunology
MH  - Sensitivity and Specificity
EDAT- 2007/02/17 09:00
MHDA- 2007/03/16 09:00
CRDT- 2007/02/17 09:00
PHST- 2007/02/17 09:00 [pubmed]
PHST- 2007/03/16 09:00 [medline]
PHST- 2007/02/17 09:00 [entrez]
AID - MYC1306 [pii]
AID - 10.1111/j.1439-0507.2006.01306.x [doi]
PST - ppublish
SO  - Mycoses. 2007 Jan;50(1):41-7. doi: 10.1111/j.1439-0507.2006.01306.x.