PMID- 17302907
OWN - NLM
STAT- MEDLINE
DCOM- 20070507
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 147
IP  - 3
DP  - 2007 Mar
TI  - Treatment of autoimmune diseases in MRL/lpr mice by allogenic bone marrow 
      transplantation plus adult thymus transplantation.
PG  - 555-63
AB  - MRL/lpr mice (H-2(k)) with Fas gene mutation develop severe autoimmune diseases, 
      and their haematolymphoid cells such as bone marrow and spleen cells showed a low 
      apoptotic activity by irradiation. Therefore, conventional bone marrow 
      transplantation (BMT) cannot be used to treat autoimmune diseases in these mice 
      (chimeric resistance). In the present study, we examine the effects of additional 
      adult thymus transplantation (TT) from the same donor on successful BMT. When the 
      MRL/lpr mice were lethally irradiated (9 x 5Gy) and reconstituted with 3 x 10(7) 
      of C57BL/6 mouse (H-2b) bone marrow cells (BMCs) in conjunction with TT, the mice 
      significantly survived long term and showed a high donor-derived chimerism in 
      comparison with those treated with BMT alone. Interestingly, the numbers of not 
      only donor-derived T cells but also B cells increased significantly in the mice 
      treated with BMT plus TT, even at the early phase of BMT. The number of aberrant 
      CD3+B220+ cells decreased significantly, and the numbers of lymphocyte subsets 
      were also normalized 4 weeks after the treatment. Finally, the autoimmune 
      diseases in MRL/lpr mice could be cured by BMT with TT. These results indicate 
      that the combination of BMT plus TT can overcome the chimeric resistance and 
      treat the autoimmune diseases in MRL/lpr mice.
FAU - Hosaka, N
AU  - Hosaka N
AD  - First Department of Pathology, Kansai Medical University, Moriguchi, Osaka, 
      Japan.
FAU - Ryu, T
AU  - Ryu T
FAU - Miyake, T
AU  - Miyake T
FAU - Cui, W
AU  - Cui W
FAU - Nishida, T
AU  - Nishida T
FAU - Takaki, T
AU  - Takaki T
FAU - Inaba, M
AU  - Inaba M
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Autoantibodies)
SB  - IM
MH  - Animals
MH  - Apoptosis/radiation effects
MH  - Autoantibodies/blood
MH  - Autoimmune Diseases/immunology/pathology/*therapy
MH  - Bone Marrow Cells/pathology/radiation effects
MH  - Bone Marrow Transplantation/immunology/*methods/pathology
MH  - Female
MH  - Lymphocyte Subsets/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred MRL lpr
MH  - Spleen/pathology/radiation effects
MH  - Survival Rate
MH  - Thymus Gland/*transplantation
MH  - Transplantation Chimera
MH  - Treatment Outcome
PMC - PMC1810495
EDAT- 2007/02/17 09:00
MHDA- 2007/05/08 09:00
PMCR- 2008/03/01
CRDT- 2007/02/17 09:00
PHST- 2007/02/17 09:00 [pubmed]
PHST- 2007/05/08 09:00 [medline]
PHST- 2007/02/17 09:00 [entrez]
PHST- 2008/03/01 00:00 [pmc-release]
AID - CEI3310 [pii]
AID - 10.1111/j.1365-2249.2006.03310.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2007 Mar;147(3):555-63. doi: 10.1111/j.1365-2249.2006.03310.x.