PMID- 17303684
OWN - NLM
STAT- MEDLINE
DCOM- 20070702
LR  - 20200930
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 292
IP  - 5
DP  - 2007 May
TI  - Divergent response of metabolite transport proteins in human skeletal muscle 
      after sprint interval training and detraining.
PG  - R1970-6
AB  - Skeletal muscle primarily relies on carbohydrate (CHO) for energy provision 
      during high-intensity exercise. We hypothesized that sprint interval training 
      (SIT), or repeated sessions of high-intensity exercise, would induce rapid 
      changes in transport proteins associated with CHO metabolism, whereas changes in 
      skeletal muscle fatty acid transporters would occur more slowly. Eight active men 
      (22 +/- 1 yr; peak oxygen uptake = 50 +/- 2 ml.kg(-1).min(-1)) performed 4-6 x 30 
      s all-out cycling efforts with 4-min recovery, 3 days/wk for 6 wk. Needle muscle 
      biopsy samples (vastus lateralis) were obtained before training (Pre), after 1 
      and 6 wk of SIT, and after 1 and 6 wk of detraining. Muscle oxidative capacity, 
      as reflected by the protein content of cytochrome c oxidase subunit 4 (COX4), 
      increased by approximately 35% after 1 wk of SIT and remained higher compared 
      with Pre, even after 6 wk of detraining (P < 0.05). Muscle GLUT4 content 
      increased after 1 wk of SIT and remained approximately 20% higher compared with 
      baseline during detraining (P < 0.05). The monocarboxylate tranporter (MCT) 4 was 
      higher after 1 and 6 wk of SIT compared with Pre, whereas MCT1 increased after 6 
      wk of training and remained higher after 1 wk of detraining (P < 0.05). There was 
      no effect of training or detraining on the muscle content of fatty acid 
      translocase (FAT/CD36) or plasma membrane associated fatty acid binding protein 
      (FABPpm) (P > 0.05). We conclude that short-term SIT induces rapid increases in 
      skeletal muscle oxidative capacity but has divergent effects on proteins 
      associated with glucose, lactate, and fatty acid transport.
FAU - Burgomaster, Kirsten A
AU  - Burgomaster KA
AD  - Exercise Metabolism Research Group, Dept of Kinesiology, McMaster Univ, Hamilton, 
      Ontario, Canada.
FAU - Cermak, Naomi M
AU  - Cermak NM
FAU - Phillips, Stuart M
AU  - Phillips SM
FAU - Benton, Carley R
AU  - Benton CR
FAU - Bonen, Arend
AU  - Bonen A
FAU - Gibala, Martin J
AU  - Gibala MJ
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070215
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Carrier Proteins)
RN  - 0 (Muscle Proteins)
SB  - IM
MH  - Carrier Proteins/*metabolism
MH  - Exercise/*physiology
MH  - Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Muscle Proteins/genetics/metabolism
MH  - Muscle, Skeletal/*metabolism
MH  - Time Factors
EDAT- 2007/02/17 09:00
MHDA- 2007/07/03 09:00
CRDT- 2007/02/17 09:00
PHST- 2007/02/17 09:00 [pubmed]
PHST- 2007/07/03 09:00 [medline]
PHST- 2007/02/17 09:00 [entrez]
AID - 00503.2006 [pii]
AID - 10.1152/ajpregu.00503.2006 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2007 May;292(5):R1970-6. doi: 
      10.1152/ajpregu.00503.2006. Epub 2007 Feb 15.