PMID- 17303690
OWN - NLM
STAT- MEDLINE
DCOM- 20070521
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 53
IP  - 4
DP  - 2007 Apr
TI  - Hyperhomocysteinemia and myocardial expression of brain natriuretic peptide in 
      rats.
PG  - 773-80
AB  - BACKGROUND: Hyperhomocysteinemia (HHcy) has been linked to impaired left 
      ventricular function and clinical class in patients with chronic heart failure. 
      We hypothesized that HHcy stimulates myocardial brain natriuretic peptide (BNP) 
      expression and induces adverse left ventricular remodeling. METHODS: We 
      randomized 50 rats into 5 groups. Groups Co1 and Co2 (controls) received a 
      typical diet. Groups Meth, Hcy1, and Hcy2 were fed the same diet supplemented 
      with 2.4% methionine, 1% homocystine, and 2% homocystine, respectively. After 12 
      weeks, we measured total plasma homocysteine (tHcy) and BNP in plasma and tissue, 
      and we performed histomorphometric analyses. RESULTS: All animals had comparable 
      baseline body weight [mean (SD) 234 (26) g] and total circulating Hcy [4.7 (1.7) 
      micromol/L]. After 12 weeks of treatment, total circulating Hcy increased in 
      Meth, Hcy1, and Hcy2 [27.3 (8.8), 40.6 (7.0), and 54.0 (46.0) micromol/L, 
      respectively] and remained unchanged in Co1 and Co2. Serum BNP significantly 
      increased in 1 of 10 animals in Meth, 3 of 10 animals in Hcy1, and 3 of 10 
      animals in Hcy2. Median (25th-75th percentile) BNP tissue concentrations in Hcy1 
      and Hcy2 were 55% higher than in the corresponding controls [Co1 vs Hcy1, 225 
      (186-263) vs 338 (262-410) pg/mg protein, P = 0.05; Co2 vs Hcy2, 179 (107-261) vs 
      308 (192-429) pg/mg protein, P = 0.12]. In the Meth group, BNP expression was 
      comparable to that of controls [200 (159-235) vs 225 (186-263) pg/mg protein, P = 
      0.32]. The percentage of perivascular and interstitial collagen and mast cell 
      infiltration were comparable in all groups, indicating no adverse cardiac 
      remodeling. CONCLUSION: Three months of intermediate HHcy stimulated increased 
      cardiac BNP expression that was not accompanied by adverse cardiac remodeling.
FAU - Herrmann, Markus
AU  - Herrmann M
AD  - Department of Clinical Chemistry, University Hospital of Saarland, Homburg/Saar, 
      Germany.
FAU - Taban-Shoma, Omid
AU  - Taban-Shoma O
FAU - Hubner, Ulrich
AU  - Hubner U
FAU - Pexa, Anette
AU  - Pexa A
FAU - Kilter, Heiko
AU  - Kilter H
FAU - Umanskaya, Natalia
AU  - Umanskaya N
FAU - Straub, Rainer Hans
AU  - Straub RH
FAU - Bohm, Michael
AU  - Bohm M
FAU - Herrmann, Wolfgang
AU  - Herrmann W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070215
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
SB  - IM
MH  - Animals
MH  - Female
MH  - Hyperhomocysteinemia/complications/*metabolism
MH  - Mast Cells/pathology
MH  - Myocardium/*metabolism/pathology
MH  - Natriuretic Peptide, Brain/*biosynthesis
MH  - Organ Size
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Ventricular Remodeling
EDAT- 2007/02/17 09:00
MHDA- 2007/05/22 09:00
CRDT- 2007/02/17 09:00
PHST- 2007/02/17 09:00 [pubmed]
PHST- 2007/05/22 09:00 [medline]
PHST- 2007/02/17 09:00 [entrez]
AID - clinchem.2006.077859 [pii]
AID - 10.1373/clinchem.2006.077859 [doi]
PST - ppublish
SO  - Clin Chem. 2007 Apr;53(4):773-80. doi: 10.1373/clinchem.2006.077859. Epub 2007 
      Feb 15.