PMID- 17304616
OWN - NLM
STAT- MEDLINE
DCOM- 20070607
LR  - 20070221
IS  - 0233-111X (Print)
IS  - 0233-111X (Linking)
VI  - 47
IP  - 1
DP  - 2007 Feb
TI  - Identification of bacteria isolated from diseased Neungee mushroom, Sarcodon 
      aspratus.
PG  - 31-9
AB  - As the first step in an investigation of the problem with quality deterioration 
      seen in the Neungee mushroom (Sarcodon aspratus) due to bacterial overgrowth 
      during its storage, an attempt to isolate bacterial strains was made using 
      infected gills of Sarcodon aspratus. Five bacterial strains were isolated; one 
      phototrophic cyanobacterial species and four heterotrophic Gram negative rods. 
      The four heterotrophic bacterial isolates (strains P, S, R, and MK1) were 
      subjected to identification based on biochemical characteristics using the Biolog 
      system, cellular fatty acid analysis using the MIDI system, cytology by scanning 
      microscopy, and 16s rDNA sequence analysis. A slow grower, the P strain (ca. 0.7 
      microm x 1.5 microm), which forms pink colonies on Tryptic Soy agar (TSA) and 
      glucose minimal salt medium containing thiamine (MT medium), belongs to genus 
      Methylobacterium, and is likely M. radiotolerans. The methanol-utilizing capacity 
      of the P strain was confirmed by growth on methanol-supplemented medium as a sole 
      carbon source. Both the S and R strains (ca. 0.5 microm x 0.8 microm) produced 
      smooth and slightly rough white colonies, respectively, on TSA, MT, and potato 
      dextrose (PD) agar are members of the Burkholderia cepacia complex. Although both 
      strains showed some differences from each other in colony morphology, nitrogen 
      fixation capacity, and denitrification, they were considered to be Burkholderia 
      stabilis because their 16s rDNA sequences showed 99.93% similarity with those of 
      B. stabilis LMG 14294T (NCBI AF 148554). The MK1 strain, a rod-shaped bacterium 
      with a tapered end (ca. 0.6 microm x 1.8 microm), produces a copious mucoid 
      substance on MT and PD agar, but not on TSA. Despite extensive identification 
      studies, the M strain is not currently identifiable, which suggests that it is a 
      novel bacterium.
FAU - Lee, Young Nam
AU  - Lee YN
AD  - Division of Forest Resources, Chungbuk National University, Cheongju, Chungbuk, 
      Republic of Korea. ynlee@chungbuk.ac.kr
FAU - Koo, Chang-Duck
AU  - Koo CD
LA  - eng
SI  - GENBANK/AY061642
SI  - GENBANK/AY061643
SI  - GENBANK/AY618904
SI  - GENBANK/AY618905
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Basic Microbiol
JT  - Journal of basic microbiology
JID - 8503885
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fatty Acids)
SB  - IM
MH  - Bacterial Typing Techniques
MH  - *Basidiomycota/metabolism/ultrastructure
MH  - Burkholderia/classification/*isolation & purification
MH  - DNA, Bacterial/metabolism
MH  - DNA, Ribosomal/metabolism
MH  - Fatty Acids/analysis
MH  - Methylobacterium/classification/*isolation & purification
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Sequence Homology, Nucleic Acid
EDAT- 2007/02/17 09:00
MHDA- 2007/06/08 09:00
CRDT- 2007/02/17 09:00
PHST- 2007/02/17 09:00 [pubmed]
PHST- 2007/06/08 09:00 [medline]
PHST- 2007/02/17 09:00 [entrez]
AID - 10.1002/jobm.200610151 [doi]
PST - ppublish
SO  - J Basic Microbiol. 2007 Feb;47(1):31-9. doi: 10.1002/jobm.200610151.