PMID- 17306467
OWN - NLM
STAT- MEDLINE
DCOM- 20070626
LR  - 20161124
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 145
IP  - 2
DP  - 2007 Mar 16
TI  - Regulation of glycinergic and GABAergic synaptogenesis by brain-derived 
      neurotrophic factor in developing spinal neurons.
PG  - 484-94
AB  - Brain-derived neurotrophic factor (BDNF) effects on the establishment of 
      glycinergic and GABAergic transmissions in mouse spinal neurons were examined 
      using combined electrophysiological and calcium imaging techniques. BDNF (10 
      ng/ml) caused a significant acceleration in the onset of synaptogenesis without 
      large effects on the survival of these neurons. Amplitude and frequency of 
      spontaneous inhibitory postsynaptic currents (sIPSCs) and miniature inhibitory 
      postsynaptic currents (mIPSCs) associated to activation of glycine and GABA(A) 
      receptors were augmented in neurons cultured with BDNF. The neurotrophin effect 
      was blocked by long term tetrodotoxin (TTX) addition suggesting a dependence on 
      neuronal activity. In addition, BDNF caused a significant increase in glycine- 
      and GABA-evoked current densities that partly explains the increase in synaptic 
      transmission. Presynaptic mechanisms were also involved in BDNF effects since 
      triethylammonium(propyl)-4-(2-(4-dibutylamino-phenyl)vinyl)pyridinium (FM1-43) 
      destaining with high K(+) was augmented in neurons incubated with the 
      neurotrophin. The effects of BDNF were mediated by receptor tyrosine kinase B 
      (TrkB) and mitogen-activated protein kinase kinase (MEK) activation since 
      culturing neurons with either 
      (9S,10R,12R)-2,3,9,10,11,12-hexahydro-10-hydroxy-9-methyl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg:3',2',1'- 
      kl]pyrrolo[3,4-i][1,6]benzodiazocine-10-carboxylic acid methyl ester (K252a) or 
      2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059) blocked the 
      augmentation in synaptic activity induced by the neurotrophin.
FAU - Carrasco, M A
AU  - Carrasco MA
AD  - Laboratory of Neurophysiology, Department of Physiology, University of 
      Concepcion, P.O. Box 160-C, Concepcion, Chile.
FAU - Castro, P
AU  - Castro P
FAU - Sepulveda, F J
AU  - Sepulveda FJ
FAU - Tapia, J C
AU  - Tapia JC
FAU - Gatica, K
AU  - Gatica K
FAU - Davis, M I
AU  - Davis MI
FAU - Aguayo, L G
AU  - Aguayo LG
LA  - eng
GR  - AA15150/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070215
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (FM1 43)
RN  - 0 (Pyridinium Compounds)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Receptors, GABA-A)
RN  - 0 (Receptors, Glycine)
RN  - 0 (Sodium Channel Blockers)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 1)
RN  - EC 2.7.12.2 (Map2k1 protein, mouse)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Glycine/*metabolism
MH  - Inhibitory Postsynaptic Potentials/drug effects/physiology
MH  - MAP Kinase Kinase 1/antagonists & inhibitors/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neural Inhibition/drug effects/physiology
MH  - Neural Pathways/drug effects/*embryology/metabolism
MH  - Neuronal Plasticity/drug effects/physiology
MH  - Neurons/*metabolism/ultrastructure
MH  - Pyridinium Compounds
MH  - Quaternary Ammonium Compounds
MH  - Receptor, trkB/drug effects/metabolism
MH  - Receptors, GABA-A/drug effects/metabolism
MH  - Receptors, Glycine/drug effects/metabolism
MH  - Sodium Channel Blockers/pharmacology
MH  - Spinal Cord/cytology/drug effects/embryology
MH  - Synapses/drug effects/metabolism/*ultrastructure
MH  - Synaptic Transmission/drug effects/physiology
MH  - gamma-Aminobutyric Acid/*metabolism
EDAT- 2007/02/20 09:00
MHDA- 2007/06/27 09:00
CRDT- 2007/02/20 09:00
PHST- 2006/03/27 00:00 [received]
PHST- 2006/11/15 00:00 [revised]
PHST- 2006/12/05 00:00 [accepted]
PHST- 2007/02/20 09:00 [pubmed]
PHST- 2007/06/27 09:00 [medline]
PHST- 2007/02/20 09:00 [entrez]
AID - S0306-4522(06)01683-6 [pii]
AID - 10.1016/j.neuroscience.2006.12.019 [doi]
PST - ppublish
SO  - Neuroscience. 2007 Mar 16;145(2):484-94. doi: 10.1016/j.neuroscience.2006.12.019. 
      Epub 2007 Feb 15.