PMID- 17306574
OWN - NLM
STAT- MEDLINE
DCOM- 20070605
LR  - 20161020
IS  - 1089-8603 (Print)
IS  - 1089-8603 (Linking)
VI  - 16
IP  - 3
DP  - 2007 May
TI  - Influence of eNOS haplotypes on the plasma nitric oxide products concentrations 
      in hypertensive and type 2 diabetes mellitus patients.
PG  - 348-55
AB  - Endothelial nitric oxide synthase (eNOS) haplotypes are associated with 
      hypertension (HT) in patients with or without type 2 diabetes mellitus (T2DM). We 
      evaluated the association of eNOS genotypes/haplotypes with the plasma 
      concentrations of nitrite/nitrate (NO(x)), which are products of nitric oxide in 
      HT, T2DM, and T2DM+HT patients. We studied eNOS polymorphisms in the promoter 
      region (T-786C), in exon 7 (Glu298Asp), and in intron 4 (b/a) in 98 controls, 68 
      patients with HT, 66 patients with T2DM, and 86 patients with T2DM+HT. NO(x) 
      concentrations were assessed using a chemiluminescence assay. No differences were 
      found in genotype/allele distribution among groups. Genotypes were not associated 
      with NO(x) concentrations. The "C-Glu-b" haplotype was more common in controls 
      than in HT/T2DM+HT groups (21% versus 9/5%, respectively, P<0.006). This 
      haplotype was more common in HT and T2DM+HT groups among subjects with high 
      (82+/-38 and 90+/-33 microM, respectively) than with low (35+/-7 and 34+/-7 
      microM, respectively) NO(x) concentrations. Conversely, the "C-Asp-b" haplotype 
      was more common in HT/T2DM+HT groups than healthy (21/21% versus 10%, 
      respectively, P<0.006). The haplotype associated with lower risk of developing 
      hypertension is also associated with higher NO(x) levels among hypertensives. 
      Conversely, the haplotype increasing the risk of developing hypertension is 
      associated with lower NO(x) levels in hypertensives.
FAU - Sandrim, Valeria C
AU  - Sandrim VC
AD  - Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of 
      Sao Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, SP, Brazil.
FAU - de Syllos, Roger W C
AU  - de Syllos RW
FAU - Lisboa, Hugo R K
AU  - Lisboa HR
FAU - Tres, Glaucia S
AU  - Tres GS
FAU - Tanus-Santos, Jose E
AU  - Tanus-Santos JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070112
PL  - United States
TA  - Nitric Oxide
JT  - Nitric oxide : biology and chemistry
JID - 9709307
RN  - 0 (Isoenzymes)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (NOS3 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Diabetes Mellitus, Type 2/*blood/enzymology/genetics
MH  - Female
MH  - Gene Frequency
MH  - Genotype
MH  - *Haplotypes
MH  - Humans
MH  - Hypertension/*blood/enzymology/genetics
MH  - Isoenzymes/genetics/metabolism
MH  - Male
MH  - Middle Aged
MH  - Nitrates/blood
MH  - Nitric Oxide/*blood
MH  - Nitric Oxide Synthase Type III/*genetics/metabolism
MH  - Nitrites/blood
MH  - Polymorphism, Genetic
MH  - Risk Factors
EDAT- 2007/02/20 09:00
MHDA- 2007/06/06 09:00
CRDT- 2007/02/20 09:00
PHST- 2006/08/08 00:00 [received]
PHST- 2006/12/13 00:00 [revised]
PHST- 2006/12/14 00:00 [accepted]
PHST- 2007/02/20 09:00 [pubmed]
PHST- 2007/06/06 09:00 [medline]
PHST- 2007/02/20 09:00 [entrez]
AID - S1089-8603(06)00459-9 [pii]
AID - 10.1016/j.niox.2006.12.007 [doi]
PST - ppublish
SO  - Nitric Oxide. 2007 May;16(3):348-55. doi: 10.1016/j.niox.2006.12.007. Epub 2007 
      Jan 12.