PMID- 17306833
OWN - NLM
STAT- MEDLINE
DCOM- 20070412
LR  - 20220330
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 80
IP  - 14
DP  - 2007 Mar 13
TI  - Receptor activator of NF-kappaB ligand induces the expression of carbonic 
      anhydrase II, cathepsin K, and matrix metalloproteinase-9 in osteoclast precursor 
      RAW264.7 cells.
PG  - 1311-8
AB  - Interleukin-1 (IL-1) is a proinflammatory cytokine that is a potent stimulator of 
      bone resorption and an inhibitor of bone formation, whereas macrophage 
      colony-stimulating factor (M-CSF) and receptor activator of NF-kappaB (RANK) 
      ligand (RANKL) are essential and sufficient for osteoclast differentiation. 
      Recently, we showed that IL-1alpha affects mineralized nodule formation in vitro 
      and halts bone matrix turnover. We also showed that IL-1alpha stimulates 
      osteoclast formation via the interaction of RANKL with RANK by increasing M-CSF 
      and prostaglandin E(2) and decreasing osteoprotegerin. Here, we examined the 
      effects of IL-1alpha or RANKL and/or M-CSF in the presence of IL-1alpha on the 
      expression of carbonic anhydrase II (CAII), cathepsin K, matrix 
      metalloproteinase-9 (MMP-9), RANK, M-CSF receptor (c-fms), and c-fos 
      transcription factor using RAW264.7 cells as osteoclast precursors. Cells were 
      cultured for up to 14 days in 0 or 100 U/ml IL-1alpha and either 50 ng/ml RANKL, 
      10 ng/ml M-CSF, or 50 ng/ml RANKL+10 ng/ml M-CSF in the presence of 100 U/ml 
      IL-1alpha. The formation of osteoclast-like cells was estimated using 
      tartrate-resistant acid phosphatase staining. Expression of the genes coding for 
      the six proteins of interest was determined using real-time PCR, and the 
      expression of the three enzymes was examined using Western blotting or ELISA. In 
      the presence of IL-1alpha, expression of CAII, cathepsin K, and MMP-9 was 
      markedly increased in cells cultured with RANKL or M-CSF+RANKL, whereas 
      expression was difficult to detect in cells cultured with IL-1alpha alone and 
      M-CSF. RANK and c-fos expression was also increased in cells cultured with RANKL 
      or M-CSF+RANKL in the presence of IL-1alpha, whereas c-fms expression did not 
      change. These results indicate that the expression of CAII, cathepsin K, and 
      MMP-9 in RAW264.7 cells is not induced by M-CSF, but by RANKL in the presence of 
      IL-1alpha.
FAU - Fujisaki, Kyosuke
AU  - Fujisaki K
AD  - Nihon University Graduate School of Dentistry, 1-8-13 Kanda Surugadai, 
      Chiyoda-ku, Tokyo 101-8310, Japan.
FAU - Tanabe, Natsuko
AU  - Tanabe N
FAU - Suzuki, Naoto
AU  - Suzuki N
FAU - Kawato, Takayuki
AU  - Kawato T
FAU - Takeichi, Osamu
AU  - Takeichi O
FAU - Tsuzukibashi, Osamu
AU  - Tsuzukibashi O
FAU - Makimura, Masaharu
AU  - Makimura M
FAU - Ito, Koichi
AU  - Ito K
FAU - Maeno, Masao
AU  - Maeno M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070123
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Drug Combinations)
RN  - 0 (Interleukin-1alpha)
RN  - 0 (NF-kappa B)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor Activator of Nuclear Factor-kappa B)
RN  - 0 (Tnfrsf11a protein, mouse)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.22.38 (Cathepsin K)
RN  - EC 3.4.22.38 (Ctsk protein, mouse)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Animals
MH  - Carbonic Anhydrase II/genetics/*metabolism
MH  - Cathepsin K
MH  - Cathepsins/genetics/*metabolism
MH  - Cell Line
MH  - Drug Combinations
MH  - Gene Expression/drug effects
MH  - Interleukin-1alpha/pharmacology
MH  - Macrophage Colony-Stimulating Factor/pharmacology
MH  - Macrophages/cytology
MH  - Matrix Metalloproteinase 9/genetics/*metabolism
MH  - Mice
MH  - NF-kappa B/genetics/*metabolism
MH  - Osteoclasts/*drug effects/metabolism
MH  - Proto-Oncogene Proteins c-fos/genetics/metabolism
MH  - RANK Ligand/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Receptor Activator of Nuclear Factor-kappa B/genetics/metabolism
MH  - Receptor, Macrophage Colony-Stimulating Factor/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2007/02/20 09:00
MHDA- 2007/04/14 09:00
CRDT- 2007/02/20 09:00
PHST- 2006/09/05 00:00 [received]
PHST- 2006/10/31 00:00 [revised]
PHST- 2006/12/15 00:00 [accepted]
PHST- 2007/02/20 09:00 [pubmed]
PHST- 2007/04/14 09:00 [medline]
PHST- 2007/02/20 09:00 [entrez]
AID - S0024-3205(07)00031-8 [pii]
AID - 10.1016/j.lfs.2006.12.037 [doi]
PST - ppublish
SO  - Life Sci. 2007 Mar 13;80(14):1311-8. doi: 10.1016/j.lfs.2006.12.037. Epub 2007 
      Jan 23.